{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:01:15Z","timestamp":1770987675638,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1.8.012.07.N.02"],"award-info":[{"award-number":["1.8.012.07.N.02"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["FN 11869"],"award-info":[{"award-number":["FN 11869"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["FN 19225"],"award-info":[{"award-number":["FN 19225"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["OT\/13\/115"],"award-info":[{"award-number":["OT\/13\/115"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Profiling of miR\u2010200b expression and its targets (transforming growth factor [TGF]\u2013\u03b22 and ZEB2) in the surgical rabbit congenital diaphragmatic hernia (DH) model before and after tracheal occlusion (TO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010eight timed\u2010pregnant rabbits had left DH creation on gestational day (GD) 23. On GD28, 17 randomly selected fetuses had TO. We harvested fetuses at GD23, GD28, or GD30. We calculated lung\u2013to\u2013body weight ratios, processed lungs for miR\u2010200b in situ hybridization and real\u2010time quantitative polymerase chain reaction, and evaluated effects on downstream targets TGF\u2010\u03b22 or ZEB2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We obtained 16 DH fetuses (n\u00a0=\u00a07 GD28 and n\u00a0=\u00a09 GD30), 13 TO fetuses (GD30), and 38 control fetuses (n\u00a0=\u00a015 GD23, n\u00a0=\u00a011 GD28, and n\u00a0=\u00a012 GD30). Diaphragmatic hernia lungs were hypoplastic, and TO resulted in control lung\u2013to\u2013body weight ratio levels. Term miR\u2010200b\u20103p levels were significantly upregulated in the hypoplastic compared with control ipsilateral lung (1.906\u00a0\u00b1\u00a00.90 vs 0.7429\u00a0\u00b1\u00a00.44) (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). Fetal TO ipsilateral lungs displayed a variable miR\u2010200b response on in situ hybridization and polymerase chain reaction, with levels similar to control and congenital DH lungs. The TGF\u2010\u03b22 was unchanged in hypoplastic and TO lungs, and ZEB2 tended to be reduced in TO compared with DH lungs (1.79 [0.4\u20102.9] vs 0.73 [0.5\u20101.4]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hypoplastic fetal rabbit lungs display upregulation of miR\u2010200b expression although downstream targets are not different from controls. Following TO, fetal rabbit lungs display a variable miR\u2010200b response.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5318","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T09:31:35Z","timestamp":1529659895000},"page":"645-653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<scp>MicroRNA<\/scp> 200b is upregulated in the lungs of fetal rabbits with surgically induced diaphragmatic hernia"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6437-8972","authenticated-orcid":false,"given":"Mary Patrice","family":"Eastwood","sequence":"first","affiliation":[{"name":"Cluster Organ Systems, Department of Development and Regeneration, and Center for Surgical Technologies, Group Biomedical Sciences KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Deprest","sequence":"additional","affiliation":[{"name":"Cluster Organ Systems, Department of Development and Regeneration, and Center for Surgical Technologies, Group Biomedical Sciences KU Leuven  Leuven Belgium"},{"name":"Fetal Medicine Unit, Clinical Department of Obstetrics and Gynaecology University Hospitals Leuven  Leuven Belgium"},{"name":"Research Department of Maternal Fetal Medicine, Institute for Women's Health (IWH) University College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5029-7899","authenticated-orcid":false,"given":"Francesca Maria","family":"Russo","sequence":"additional","affiliation":[{"name":"Cluster Organ Systems, Department of Development and Regeneration, and Center for Surgical Technologies, Group Biomedical Sciences KU Leuven  Leuven Belgium"},{"name":"Fetal Medicine Unit, Clinical Department of Obstetrics and Gynaecology University Hospitals Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Cluster Organ Systems, Department of Development and Regeneration, and Center for Surgical Technologies, Group Biomedical Sciences KU Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew","family":"Mulhall","sequence":"additional","affiliation":[{"name":"Department of Surgery, Pediatrics &amp; Child Health University of Manitoba  Winnipeg MB Canada"},{"name":"Department of Physiology University of Manitoba  Winnipeg MB Canada"},{"name":"Biology of Breathing Children's Hospital Research Institute of Manitoba  Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Iwasiow","sequence":"additional","affiliation":[{"name":"Department of Surgery, Pediatrics &amp; Child Health University of Manitoba  Winnipeg MB Canada"},{"name":"Department of Physiology University of Manitoba  Winnipeg MB Canada"},{"name":"Biology of Breathing Children's Hospital Research Institute of Manitoba  Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas H.","family":"Mahood","sequence":"additional","affiliation":[{"name":"Department of Surgery, Pediatrics &amp; Child Health University of Manitoba  Winnipeg MB Canada"},{"name":"Department of Physiology University of Manitoba  Winnipeg MB Canada"},{"name":"Biology of Breathing Children's Hospital Research Institute of Manitoba  Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0108-3157","authenticated-orcid":false,"given":"Richard","family":"Keijzer","sequence":"additional","affiliation":[{"name":"Department of Surgery, Pediatrics &amp; Child Health University of Manitoba  Winnipeg MB Canada"},{"name":"Department of Physiology University of Manitoba  Winnipeg MB Canada"},{"name":"Biology of Breathing Children's Hospital Research Institute of Manitoba  Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420460605"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2014-306174"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2033"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.6141"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.grf.0000184774.02793.0c"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2010.10.008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2011.08.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.6450"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.22739"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201100899"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1209026"},{"key":"e_1_2_8_13_1","unstructured":"BegMS HongDS SachdevJC et al.A multicenter phase I study of MRX34 microRNA miR\u2010RX34 liposomal injection [Internet].2016. 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