{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:13:35Z","timestamp":1772118815374,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To gather additional data on the ability to detect subchromosomal abnormalities of various sizes in single fetal cells isolated from maternal blood, using low\u2010coverage shotgun next\u2010generation sequencing for cell\u2010based noninvasive prenatal testing (NIPT).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Fetal trophoblasts were recovered from approximately 30\u00a0mL of maternal blood using maternal white blood cell depletion, density\u2010based cell separation, immunofluorescence staining, and high\u2010resolution scanning. These trophoblastic cells were picked as single cells and underwent whole genome amplification for subsequent genome\u2010wide copy number analysis and genotyping to confirm the fetal origin of the cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Applying our fetal cell isolation method to a series of 125 maternal blood samples, we detected on average 4.17 putative fetal cells\/sample. The series included 15 cases with clinically diagnosed fetal aneuploidies and five cases with subchromosomal abnormalities. This method was capable of detecting findings that were 1 to 2\u00a0Mb in size, and all were concordant with the microarray or karyotype data obtained on a fetal sample. A minority of fetal cells showed evidence of genome degradation likely related to apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We demonstrate that this cell\u2010based NIPT method has the capacity to reliably diagnose fetal chromosomal abnormalities down to 1 to 2\u00a0Mb in size.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.5377","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T04:00:35Z","timestamp":1540440035000},"page":"1069-1078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Reliable detection of subchromosomal deletions and duplications using cell\u2010based noninvasive prenatal testing"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8888-8563","authenticated-orcid":false,"given":"Liesbeth","family":"Vossaert","sequence":"first","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roseen","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinming","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunjing","family":"Qu","sequence":"additional","affiliation":[{"name":"Baylor Genetics Laboratory  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Henke","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Seubert","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Chow","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lance","family":"U'ren","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brennan","family":"Enright","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackie","family":"Stilwell","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"},{"name":"Immune Design  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Kaldjian","sequence":"additional","affiliation":[{"name":"RareCyte Inc.  Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"},{"name":"Baylor Genetics Laboratory  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"},{"name":"Baylor Genetics Laboratory  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brynn","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center  New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"Wapner","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Columbia University Medical Center  New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Breman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignatia","family":"Van den Veyver","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"},{"name":"Department of Obstetrics and Gynecology Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2083-3192","authenticated-orcid":false,"given":"Arthur","family":"Beaudet","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,19]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"American College of Obstetricians and Gynecologists.ACOG committee opinion. [Internet]. American College of Obstetricians and Gynecologists; Accessed:https:\/\/www.acog.org\/Resources\u2010And\u2010Publications\/Committee\u2010Opinions\/Committee\u2010on\u2010Genetics\/Cell\u2010free\u2010DNA\u2010Screening\u2010for\u2010Fetal\u2010Aneuploidy"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000001439"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2013.11.014"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5049"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.04.005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23232"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5226"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203382"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4924"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4948"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5150"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b03073"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.347"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000053877"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-2365-x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3641"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000441294"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000071983"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.12.007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aak9787"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.5377","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.5377","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pd.5377","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.5377","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T12:43:08Z","timestamp":1694781788000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.5377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,19]]},"references-count":20,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/pd.5377"],"URL":"https:\/\/doi.org\/10.1002\/pd.5377","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.734292798.793559906","asserted-by":"object"}]},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,19]]},"assertion":[{"value":"2018-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}