{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:38:33Z","timestamp":1761745113702,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T00:00:00Z","timestamp":1573430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012230","name":"Water Resources Department of Guangdong Province","doi-asserted-by":"publisher","award":["2013B022000005","2016A030313610"],"award-info":[{"award-number":["2013B022000005","2016A030313610"]}],"id":[{"id":"10.13039\/501100012230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","award":["2014 YZ\u201000551","201504282321393","201604020078","201604020091"],"award-info":[{"award-number":["2014 YZ\u201000551","201504282321393","201604020078","201604020091"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671470"],"award-info":[{"award-number":["81671470"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2018YFC 1004104"],"award-info":[{"award-number":["2018YFC 1004104"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyze the fetal fraction, fetal sex, and chromosomal aneuploidy in multiple pregnancies using noninvasive prenatal testing (NIPT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>A total of 362 pregnant women including 203 singleton pregnancies, 69 twins, and 90 higher\u2010order multiple pregnancies were recruited. Fetal fractions estimated by size ratio\u2010based and Y chromosome\u2010based approaches in singleton pregnancies with male fetus were used as source data to establish the model. The model was then applied to multiple pregnancies for fetal fraction estimation. By comparing the fetal fractions estimated by size ratio to those estimated by Y chromosome or autosomal chromosomes, fetal sex and chromosomal aneuploidy can be analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The size ratio\u2010based approach has been well established in estimating fetal fractions for twin and higher\u2010order multiple pregnancies. Fetal fraction had a positive correlation with gestational age in twin and triplet pregnancies. Fetal sex was determined with accuracies of 98.6% (95% CI, 92.19%\u201099.96%) in twins and 97.6% (95% CI, 91.76%\u201099.71%) in triplet pregnancies. Four trisomy 21, one trisomy 18, and one trisomy 13 cases were detected by NIPT. Two trisomy 21 singleton pregnancies and one trisomy 21 twin pregnancy were confirmed by karyotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Fetal sex and chromosomal aneuploidy in multiple pregnancies can be determined using NIPT.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5597","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T16:47:08Z","timestamp":1572540428000},"page":"1273-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Validation of fetal DNA fraction estimation and its application in noninvasive prenatal testing for aneuploidy detection in multiple pregnancies"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6906-872X","authenticated-orcid":false,"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Fetal Medicine and Prenatal Diagnosis The Third Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Obstetrics &amp; Gynecology Institute of Guangzhou  Guangzhou China"},{"name":"The Medical Centre for Critical Pregnant Women in Guangzhou  Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province  Guangzhou China"},{"name":"Key Laboratory for Reproduction and Genetics of Guangdong Higher Education Institutes  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuman","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Automation Science and Engineering South China University of Technology  Guangzhou China"},{"name":"Guangzhou Jingke Medical Laboratory  Guangzhou China"},{"name":"Shenzhen Jingke Genetic Science and Technology Co., Ltd.  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulai","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangzhou Jingke Medical Laboratory  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanchen","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Fetal Medicine and Prenatal Diagnosis The Third Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Obstetrics &amp; Gynecology Institute of Guangzhou  Guangzhou China"},{"name":"The Medical Centre for Critical Pregnant Women in Guangzhou  Guangzhou China"},{"name":"Key Laboratory for Major Obstetric Diseases of Guangdong Province  Guangzhou China"},{"name":"Key Laboratory for Reproduction and Genetics of Guangdong Higher Education Institutes  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Fetal Medicine and Prenatal Diagnosis The Third Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Obstetrics &amp; 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