{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:21:47Z","timestamp":1761718907749,"version":"3.40.5"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>There are many causes of fetal effusions, including the rare lysosomal storage diseases (LSDs). Vacuolated lymphocytes (VLs) are found in the blood of infants with LSDs, and their presence in fetal effusion could increase the risk of underlying LSD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Between 2006 and 2018, all fetal effusions samples from 43 fetal multidisciplinary centers were referred to a single laboratory. Cells were counted, and, if observed, VLs were categorized and counted. Screening for LSDs was performed by metabolite analyses on amniotic fluid supernatant. The diagnosis of an LSD was confirmed by measuring the activity of the corresponding enzyme and\/or mutation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our laboratory received 614 ascitic fluids and 280 pleural fluids sampled between 22 and 33\u2009weeks of gestation. The final diagnosis was LSD in 16 cases (1.8%). VLs were reported in all these 16 cases, in a mix of lymphocytes with and without vacuoles. Vacuoles in VLs varied in size and number. In most cases, VLs were easy to recognize, with numerous, large, round, well\u2010defined vacuoles, but in three cases of LSDs, VLs were atypical.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The finding of VLs in fetal effusions is an inexpensive first\u2010line test that may help to prioritize biochemical and genetic tests for LSDs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5657","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T11:28:19Z","timestamp":1580470099000},"page":"605-611","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Finding vacuolated lymphocytes in fetal effusions improves the prenatal diagnosis of lysosomal storage diseases"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1124-1283","authenticated-orcid":false,"given":"Sarah","family":"Taconet","sequence":"first","affiliation":[{"name":"Service d'Anatomie et Cytologie Pathologiques H\u00f4pital Simone Veil  Eaubonne France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Dreux","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Hormonologie H\u00f4pital Robert\u2010Debr\u00e9, APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabien","family":"Guimiot","sequence":"additional","affiliation":[{"name":"F\u0153topathologie H\u00f4pital Robert\u2010Debr\u00e9 Paris, APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magali","family":"Pettazzoni","sequence":"additional","affiliation":[{"name":"Service de Biochimie et Biologie Mol\u00e9culaire Grand Est, UM Pathologies M\u00e9taboliques, \u00c9rythrocytaires et D\u00e9pistage P\u00e9rinatal Hospices Civils de Lyon  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bichr","family":"Allaf","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Hormonologie H\u00f4pital Robert\u2010Debr\u00e9, APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Spaggiari","sequence":"additional","affiliation":[{"name":"Service d'Obst\u00e9trique et de M\u00e9decine F\u0153tale H\u00f4pital Necker, APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8877-0381","authenticated-orcid":false,"given":"Jonathan","family":"Rosenblatt","sequence":"additional","affiliation":[{"name":"Centre Pluridisciplinaire de Diagnostic Pr\u00e9natal Robert\u2010Debr\u00e9 APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suonavy","family":"Khung\u2010Savatovsky","sequence":"additional","affiliation":[{"name":"F\u0153topathologie H\u00f4pital Robert\u2010Debr\u00e9 Paris, APHP  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4522"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.10.007"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.12.018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.3972"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32677"},{"key":"e_1_2_11_8_1","first-page":"17","article-title":"Aspect cytologique normal et pathologique du sang chez le nouveau\u2010n\u00e9 et le jeune enfant","volume":"64","author":"Fenneteau O","year":"2006","journal-title":"Ann Biol Clin"},{"key":"e_1_2_11_9_1","first-page":"483","article-title":"Valeur diagnostique du my\u00e9logramme au cours des pathologies constitutionnelles de l'enfant","volume":"65","author":"Fenneteau O","year":"2007","journal-title":"Ann Biol Clin"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.027045"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3233\/TRD-160005"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-993847"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4274"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-016-9947-8"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12170"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12288"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.5657","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.5657","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pd.5657","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.5657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:41:15Z","timestamp":1693996875000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.5657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,11]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/pd.5657"],"URL":"https:\/\/doi.org\/10.1002\/pd.5657","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"type":"print","value":"0197-3851"},{"type":"electronic","value":"1097-0223"}],"subject":[],"published":{"date-parts":[[2020,2,11]]},"assertion":[{"value":"2019-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}