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The blood samples were collected as follows: 32 from the umbilical cord of fetuses with gastroschisis, 32 from their respective mothers, 16 from the umbilical cord of fetuses without malformation, and 16 from their respective mothers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The differential DNA methylation analysis showed a significant difference between the groups. The enrichment analysis resulted in 12 sites related to T\u2010cell activation (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0128). The sites with different methylation status contained 10 genes, three of which were related to the beta\u20102\u2010microglobulin gene. The methylation profile observed in the fetuses with gastroschisis was not inherited from the mothers. In addition, there was no association between maternal urinary tract infection, smoking, and alcohol use and different methylated sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We established the methylation profile of gastroschisis fetuses, which differs from that of normal fetuses. 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