{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:44:35Z","timestamp":1790603075026,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T00:00:00Z","timestamp":1627862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Nonimmune hydrops fetalis (NIHF) has varied etiology. We assessed the etiological spectrum and evaluated the utility of fetal whole exome sequencing (fWES) for the diagnosis of NIHF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this prospective cohort study, we evaluated antenatally diagnosed fetuses with NIHF between July 2018 and December 2019 according to the routine diagnostic algorithm. Fetuses that remained undiagnosed after routine NIHF workup were subjected to fetal chromosomal microarray and\/or WES. Pregnancies were followed up for clinical outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 45 fetuses, consanguinity and recurrent hydrops fetalis were observed in 13.3% (6\/45) and 28.8% (13\/45), respectively. Overall, an etiological diagnosis was possible in 75.5% (34\/45) of fetuses, while the cause remained unknown in 24.4% (11\/45). A genetic etiology was identified in 46.6% (21\/45): aneuploidy and monogenic disorders in 28.8% (13\/45) and 17.8% (8\/45), respectively. fWES on 19 fetuses detected disease\u2010causing variants in 42.1% (8\/19). Nine novel variants were detected in\n                      <jats:italic>RAPSN<\/jats:italic>\n                      ,\n                      <jats:italic>ASCC1<\/jats:italic>\n                      ,\n                      <jats:italic>NEB<\/jats:italic>\n                      ,\n                      <jats:italic>PKD1L1<\/jats:italic>\n                      ,\n                      <jats:italic>GUSB<\/jats:italic>\n                      , and\n                      <jats:italic>PIEZO1<\/jats:italic>\n                      . Only 8.8% (4\/45) of the cohort survived without morbidity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study describes the etiological spectrum and the disease\u2010causing variants in an Indian cohort of hydropic fetuses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.6022","type":"journal-article","created":{"date-parts":[[2021,7,24]],"date-time":"2021-07-24T07:52:56Z","timestamp":1627113176000},"page":"1414-1424","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Utility of fetal whole exome sequencing in the etiological evaluation and outcome of nonimmune hydrops fetalis"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9441-9536","authenticated-orcid":false,"given":"Alec Reginald 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