{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:34:41Z","timestamp":1761154481935},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,4,24]],"date-time":"2003-04-24T00:00:00Z","timestamp":1051142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to provide a noninvasive prenatal diagnosis of the hemoglobin E (Hb E) related disorder, we have evaluated the possibility of identifying the fetal \u03b2<jats:sup>E<\/jats:sup>\u2010globin gene in maternal plasma. The analysis was performed during 8 to 18 weeks of gestation using DNA extracted from 200 \u00b5L of plasma from pregnant women whose husbands carried Hb E. The \u03b2<jats:sup>E<\/jats:sup>\u2010globin mutation in maternal plasma was detected by a nested PCR amplification followed by the <jats:italic>Mnl<\/jats:italic> I restriction analysis. The result was compared with that of routine analysis of the CVS specimens. Among the five pregnant women examined, the fetal \u03b2<jats:sup>E<\/jats:sup>\u2010globin gene was identified in maternal plasma in three of them and the result was completely concordant with the conventional CVS analysis. This simple noninvasive prenatal detection of the fetal \u03b2<jats:sup>E<\/jats:sup>\u2010globin gene should prove useful in a prevention and control program of Hb E\/\u03b2\u2010thalassemia in countries where the \u03b2<jats:sup>E<\/jats:sup>\u2010globin gene is prevalent. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pd.607","type":"journal-article","created":{"date-parts":[[2003,5,12]],"date-time":"2003-05-12T11:31:28Z","timestamp":1052739088000},"page":"393-396","source":"Crossref","is-referenced-by-count":33,"title":["Prenatal detection of fetal hemoglobin E gene from maternal plasma"],"prefix":"10.1002","volume":"23","author":[{"given":"Goonnapa","family":"Fucharoen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warunee","family":"Tungwiwat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thawalwong","family":"Ratanasiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanokwan","family":"Sanchaisuriya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supan","family":"Fucharoen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/clinchem\/46.2.301","article-title":"Prenatal diagnosis of myotonic dystrophy using fetal DNA obtained from maternal plasma","volume":"46","author":"Amicucci P","year":"2000","journal-title":"Clin Chem"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(76)80800-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1093\/clinchem\/48.5.778","article-title":"Noninvasive prenatal diagnosis exclusion of congenital adrenal hyperplasia by maternal plasma analysis: a feasibility study","volume":"48","author":"Chiu RWK","year":"2002","journal-title":"Clin Chem"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11086-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)92147-R"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(94)90242-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00210668"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630268709036587"},{"key":"e_1_2_1_10_1","first-page":"16","article-title":"Prenatal diagnosis of thalassemia and hemoglobinopathies in Thailand: experience from 100 pregnancies","volume":"22","author":"Fucharoen S","year":"1991","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.439"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-001-0649-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269708997381"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_2_1_15_1","first-page":"174","article-title":"Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma","volume":"10","author":"Lo YMD","year":"1998","journal-title":"N Engl J Med"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390051008"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02767-7"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(01)00250-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.24.2.110"},{"key":"e_1_2_1_20_1","first-page":"31","volume-title":"The Thalassaemias","author":"Wood WG","year":"1983"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.607","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.607","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T02:12:47Z","timestamp":1700273567000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,24]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1002\/pd.607"],"URL":"https:\/\/doi.org\/10.1002\/pd.607","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,24]]}}}