{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:50:46Z","timestamp":1768319446912,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T00:00:00Z","timestamp":1674950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Fetal surgery for spina bifida aperta (SBA) by open hysterotomy typically repairs anatomical native tissue in layers. Increasingly, fetoscopic repair is performed using a dural patch followed by skin closure. We studied the host response to selected commercially available patches currently being used in a fetal rabbit model for spina bifida repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SBA was surgically induced at 23\u201324\u00a0days of gestation (term\u00a0=\u00a031\u00a0days). Fetal rabbits were assigned to unrepaired (SBA group), or immediate repair with Duragen\u2122 or Durepair\u2122. Non\u2010operated littermates served as normal controls. At term, spinal cords underwent immunohistochemical staining including Nissl and glial fibrillary acidic protein. We hypothesized that spinal cord coverage with a dural patch and skin closure would preserve motor neuron density within the non\u2010inferiority limit of 201.65\u00a0cells\/mm<jats:sup>2<\/jats:sup> and reduce inflammation compared to unrepaired SBA fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Motor neuron density assessed by Nissl staining was conserved both by Duragen (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 89.5; 95% CI \u2212158.3 to \u221220.6) and Durepair (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 37.0; 95% CI \u2212132.6 to \u221258.5), whereas density of GFAP\u2010positive cells to quantify inflammation was lower than in unrepaired SBA\u2010fetuses (SBA 2366.0\u00a0\u00b1\u00a0669.7\u00a0cells\/mm<jats:sup>2<\/jats:sup> vs. Duragen 1274.0\u00a0\u00b1\u00a0157.2\u00a0cells\/mm<jats:sup>2<\/jats:sup>; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0002, Durepair 1069.0\u00a0\u00b1\u00a0270.7\u00a0cells\/mm<jats:sup>2<\/jats:sup>; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Covering the rabbit spinal cord with either Duragen or Durepair followed by skin closure preserves motor neuron density and reduces the inflammatory response.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.6315","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T04:12:31Z","timestamp":1674015151000},"page":"370-381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Local host response of commercially available dural patches for fetal repair of spina bifida aperta in rabbit model"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-1414","authenticated-orcid":false,"given":"Yada","family":"Kunpalin","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration Cluster Woman and Child, Biomedical Sciences MyFetUZ Fetal Research Center KU Leuven  Leuven Belgium"},{"name":"Elizabeth Garrett Anderson Institute for Women's Health University College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simen","family":"Vergote","sequence":"additional","affiliation":[{"name":"Department of Development and Regeneration Cluster Woman and Child, Biomedical Sciences MyFetUZ Fetal Research Center KU Leuven  Leuven Belgium"},{"name":"Department of Obstetrics and Gynaecology University Hospitals Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7331-6354","authenticated-orcid":false,"given":"Luc","family":"Joyeux","sequence":"additional","affiliation":[{"name":"Division of Pediatric Surgery Michael E. 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