{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:16:37Z","timestamp":1775765797783,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Long cell\u2010free DNA (cfDNA) can be found in the plasma of pregnant women and cancer patients. We investigated if droplet digital PCR (ddPCR) can analyze such molecules for diagnostic purposes using preeclampsia as a model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Plasma samples from ten preeclamptic and sixteen normal pregnancies were analyzed. Two ddPCR assays targeting a single\u2010copy gene, VCP, and one ddPCR assay targeting LINE\u20101 repetitive regions were used to measure the percentages of long cfDNA &gt;533, 1001, and 170 bp, respectively. The LINE\u20101 assay was developed as guided by in silico PCR analyses to better differentiate preeclamptic and normal pregnancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Preeclamptic patients had a significantly lower median percentage of long cfDNA than healthy pregnant controls, as determined by the LINE\u20101 170 bp assay (28.9% vs. 35.1%,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.0001) and the VCP 533 bp assay (6.6% vs. 8.7%,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.014). The LINE\u20101 assay provided a better differentiation than the VCP 533 bp assay (area under ROC curves, 0.94 vs. 0.79).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>ddPCR is a cost\u2010effective approach for unlocking diagnostic information carried by long cfDNA in plasma and may have applications for the detection of preeclampsia. Further longitudinal studies with larger cohorts are required to assess the clinical utility of this test.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.6432","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T04:15:44Z","timestamp":1693541744000},"page":"1385-1393","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Droplet digital PCR is a cost\u2010effective method for analyzing long cell\u2010free DNA in maternal plasma: Application in preeclampsia"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9097-9141","authenticated-orcid":false,"given":"Wanxia","family":"Gai","sequence":"first","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8207-4641","authenticated-orcid":false,"given":"Stephanie C. Y.","family":"Yu","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. T. Charlotte","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlei","family":"Peng","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9153-0343","authenticated-orcid":false,"given":"Tak Y.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peiyong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"},{"name":"State Key Laboratory of Translational Oncology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. C. Allen","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"},{"name":"State Key Laboratory of Translational Oncology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8746-0293","authenticated-orcid":false,"given":"Y. M. Dennis","family":"Lo","sequence":"additional","affiliation":[{"name":"Centre for Novostics Hong Kong Science Park Pak Shek Kok  New Territories Hong Kong SAR China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Shatin Hong Kong SAR China"},{"name":"Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"},{"name":"State Key Laboratory of Translational Oncology The Chinese University of Hong Kong Prince of Wales Hospital  Shatin Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw3616"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001720"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1804134115"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159\u20108290.cd\u201019\u20100622"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.261396.120"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406103111"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2016.254813"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201039076\u20107"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.06.015"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2114937118"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvac180"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.11.050"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1615800113"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.024893"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.04.017"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa1704559"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.6432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pd.6432","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.6432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T11:13:49Z","timestamp":1773314029000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.6432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,1]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1002\/pd.6432"],"URL":"https:\/\/doi.org\/10.1002\/pd.6432","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,1]]},"assertion":[{"value":"2023-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}