{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:31:04Z","timestamp":1759336264912,"version":"3.40.5"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess whether the fetal fraction (FF) has an impact on the screen\u2010positive rate (SPR) in cell\u2010free DNA (cfDNA) screening for trisomy 21.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Retrospective analysis based on samples analyzed using the Harmony<jats:sup>\u00ae<\/jats:sup> Prenatal Test (Roche Inc). Due to the size of the data set, we focused on the SPR, which was stratified according to the maternal age, weight, gestational age, and FF distribution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study cohort consisted of 364,881 patients, including 2614 with a high\u2010risk\u2010result. Median maternal and gestational ages were 34.6\u00a0years and 12.4\u00a0weeks. FF was dependent on maternal age, weight, and gestational age. SPR was 0.72% and it was independent of maternal weight but was dependent on maternal age. There was a positive but weak association between the FF and the SPR until the FF reached 20.0% (OR <jats:italic>p<\/jats:italic>\u00a0=\u00a01.021, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001, Nagelkerkes <jats:italic>r<\/jats:italic>2\u00a0=\u00a00.001). This group included 357,800 pregnancies or 98.1% of the study population. In the group of pregnancies with a FF &gt; 20%, the association was stronger (OR 1.099, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001, Nagelkerkes <jats:italic>r<\/jats:italic>2\u00a0=\u00a00.042).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The SPR in cfDNA screening for trisomy 21 was relatively constant up to a FF of about 20%.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.6448","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T05:41:36Z","timestamp":1696398096000},"page":"1536-1543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Screen\u2010positive rate in cell free DNA screening for trisomy 21"],"prefix":"10.1002","volume":"43","author":[{"given":"Kai","family":"L\u00fcthgens","sequence":"first","affiliation":[{"name":"Cenata GmbH  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"H\u00e4big","sequence":"additional","affiliation":[{"name":"Cenata GmbH  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiri","family":"Sonek","sequence":"additional","affiliation":[{"name":"Fetal Medicine Foundation USA  Dayton Ohio USA"},{"name":"Division of Maternal Fetal Medicine Wright State University  Dayton Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1497-5143","authenticated-orcid":false,"given":"Karl Oliver","family":"Kagan","sequence":"additional","affiliation":[{"name":"Department of Women's Health University Women's Hospital  T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.17484"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen\u20102015\u2010010002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11164760"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404\u2010022\u201006477\u20105"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.17483"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26106"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.3.421"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1700"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5620"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4625"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14694"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4366"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.6328"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000367626"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2922"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.01.030"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000484317"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20100648\u20105374"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5182"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.6448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T08:35:38Z","timestamp":1700210138000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.6448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,3]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/pd.6448"],"URL":"https:\/\/doi.org\/10.1002\/pd.6448","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"type":"print","value":"0197-3851"},{"type":"electronic","value":"1097-0223"}],"subject":[],"published":{"date-parts":[[2023,10,3]]},"assertion":[{"value":"2023-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}