{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T23:56:00Z","timestamp":1700265360218},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2003,8,12]],"date-time":"2003-08-12T00:00:00Z","timestamp":1060646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of the study was to assess the feasibility of analysis of fetal nucleated red blood cells (NRBC) present in the maternal circulation by laser\u2010scanning cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CD71\u2010positive cells were obtained by magnetic cell sorting of peripheral blood of pregnant women after density centrifugation. Immunofluorescence for the Hb\u03b3\u2010chain was combined with fluorescent staining of DNA (TO\u2010PRO\u20103) and fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) with a Y\u2010chromosome specific probe. The cells were scanned on a slide using a laser\u2010scanning cytometer (LSC). Events double positive for Hb\u03b3 and TO\u2010PRO\u20103 were relocated and their morphology and FISH reactivity were visually assessed. Determination of male fetal sex with LSC was compared with findings from amniocentesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 8\/15 pregnancies with male fetuses and in 0\/9 with females (apart from one case with a male\/female twin pregnancy), we detected Y\u2010chromosome\u2010positive NRBC. In pregnancies with female fetuses, Y\u2010chromosome\u2010positive cells other than NRBC were found in all women who had previously given birth to male babies, whereas women with no abortion and no male babies in their history did not present with Y\u2010chromosome\u2010positive non\u2010NRBC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>On the basis of automatic relocation of once\u2010defined cells of fetal origin from the current pregnancy, laser\u2010scanning cytometry is likely to facilitate repeated (poly\u2010)FISH analysis and single\u2010cell PCR for noninvasive prenatal diagnosis. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.668","type":"journal-article","created":{"date-parts":[[2003,9,11]],"date-time":"2003-09-11T13:22:05Z","timestamp":1063286525000},"page":"710-715","source":"Crossref","is-referenced-by-count":12,"title":["Fetal nucleated red blood cells in peripheral blood of pregnant women: detection and determination of location on a slide using laser\u2010scanning cytometry"],"prefix":"10.1002","volume":"23","author":[{"given":"Simone","family":"Hennerbichler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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