{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:15:17Z","timestamp":1759968917637,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Prenatal cfDNA screening is the most sensitive and specific screen for common aneuploidies in singleton and twin pregnancies and has been endorsed by several professional societies as a first\u2010tier screen or contingent screen. However, data for triplet pregnancies is lacking, as these pregnancies are relatively uncommon and obtaining sufficient data for a robust analysis of test performance is challenging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This study presents a retrospective review of over 1500 samples from triplet pregnancies screened via cfDNA for common aneuploidies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean patient age was 34\u00a0years, while mean gestational age was 13\u00a0weeks. The most common indication for testing was patient age, representing &gt;\u00a060% of the cohort. There were 13 screen\u2010positive cases (1.01%), 270 NR cases (17.32%), and the remainder were screen\u2010negative. Complete or partial genetic and\/or obstetric outcome information (including birth and neonatal outcomes) was available for 147 samples, including all 13 positive cfDNA samples. No false positive or false negative cases were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The data from this study support the notion that cfDNA screening in triplet pregnancies is a reasonable approach given the lack of alternative screening options for these patients and that the performance likely approaches that of twin pregnancies, albeit with a higher no\u2010call rate.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.6812","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T22:14:22Z","timestamp":1746742462000},"page":"705-712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Laboratory Experience With Prenatal cfDNA Screening in Triplet Pregnancies"],"prefix":"10.1002","volume":"45","author":[{"given":"Erica","family":"Soster","sequence":"first","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittany","family":"Dyr","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savannah","family":"Moore","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"},{"name":"Wake Forest University  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eyad","family":"Almasri","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hany","family":"Magharyous","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan\u2010Sebastian","family":"Saldivar","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3491-0841","authenticated-orcid":false,"given":"Phillip","family":"Cacheris","sequence":"additional","affiliation":[{"name":"Labcorp  San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.3892"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/GRF.0000000000000814"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000004397"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000004084"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11071937"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5832"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.6548"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5597"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/aog.0000000000004385"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0220979"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057381"},{"volume-title":"Poster Presentation Presented at: The American College of Medical Genetics Annual Clinical Genetics Meeting","year":"2017","author":"Mazloom A. 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