{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:45:51Z","timestamp":1791877551152,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Prenatal cell\u2010free DNA screening is widely used to assess the risk of fetal trisomy 21, 18, and 13. Although commercial and laboratory\u2010developed tests (LDTs) show high accuracy in validation studies, real\u2010world longitudinal performance data are lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>In Belgium, prenatal cell\u2010free DNA screening is a population screening offered to all pregnant people, regardless of their a priori risk. Over 3\u00a0years, all confirmatory diagnostic tests after a positive prenatal cell\u2010free DNA screening result were recorded along with the technologies used and their outcomes. Positive predictive values (PPVs) for the three common fetal trisomies were calculated per technology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 1158 diagnostic tests were performed after a positive prenatal cell\u2010free DNA screening. Of these, 58.5% came from an LDT, 15.7% from VeriSeq (Illumina), 6.6% from Harmony (Roche), 5% from Vanadis (Perkin\u2010Elmer); for 14.2% the technology could not be traced. PPVs ranged from 92.2% to 65.7% for trisomy 21, from 82.8% to 44.4% for trisomy 18, and from 40% to 18.2% for trisomy 13. Overall, the LDT performed better than the commercial tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study confirms the reported accuracy of commercial tests and LDT, while showing that LDT generally outperforms commercial tests. Despite high specificities across tests, differences in PPVs significantly impact pregnant people and the national health care system.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.70236","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:15:17Z","timestamp":1786990517000},"page":"1771-1777","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multicentric Longitudinal Performance Monitoring of Different Prenatal Cell\u2010Free DNA Screening Technologies in Belgium"],"prefix":"10.1002","volume":"46","author":[{"given":"Armelle","family":"Duquenne","sequence":"first","affiliation":[{"name":"Center for Human Genetics Cliniques Universitaires Saint\u2010Luc UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Brison","sequence":"additional","affiliation":[{"name":"Center for Human Genetics University Hospital 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(2022): [cited 2023 Dec 8] https:\/\/support.illumina.com\/content\/dam\/illumina\u2010support\/documents\/documentation\/chemistry_documentation\/veriseq\u2010nipt\u2010v2\/1000000078751_08_veriseq\u2010nipt\u2010solution\u2010v2\u2010package\u2010insert.pdf."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvab067"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.919855"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4686"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.05.021"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa1407349"},{"key":"e_1_2_11_10_1","unstructured":"PerkinElmer inc. now R.Vanadis NIPT System[Internet]. 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