{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:45:50Z","timestamp":1791877550331,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2705500"],"award-info":[{"award-number":["2023YFC2705500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82130046"],"award-info":[{"award-number":["82130046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82320108009"],"award-info":[{"award-number":["82320108009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82501995"],"award-info":[{"award-number":["82501995"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["25Y12800300"],"award-info":[{"award-number":["25Y12800300"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["23Y11901400"],"award-info":[{"award-number":["23Y11901400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess concordance between cell\u2010free DNA screening and invasive prenatal diagnosis after single euploid embryo transfer following pre\u2010implantation genetic testing for aneuploidy (PGT\u2010A), and estimate residual chromosomal risk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      This retrospective cohort included 1079 singleton ongoing pregnancies after PGT\u2010A between August 2019 and March 2024. Prenatal testing comprised cell\u2010free DNA screening alone (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0317), amniocentesis alone (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0500), or both tests (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0262). Chromosomal microarray analysis of amniocytes was the reference standard.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the dual\u2010testing cohort, eight pregnancies had high\u2010risk cell\u2010free DNA results, of which one was confirmed as 47, XXX. One low\u2010risk result was subsequently diagnosed as sex chromosome mosaicism. Sensitivity, specificity, positive predictive value and negative predictive value were 50.0% (95% CI, 2.7\u201397.3), 97.3% (94.3\u201398.8), 12.5% (0.6\u201353.3) and 99.6% (97.5\u201399.9), respectively. Among 762 pregnancies undergoing amniocentesis, residual confirmed aneuploidy risk after euploid embryo transfer was 0.1% (1\/762). The overall yield of clinically relevant CMA findings was 0.5% (4\/762), including two pathogenic microdeletions. One pregnancy loss within 7\u00a0days after amniocentesis occurred (0.1%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>After PGT\u2010A, cell\u2010free DNA screening showed high negative predictive value but limited positive predictive value. These findings support risk\u2010stratified prenatal testing, while invasive diagnosis remains indicated after high\u2010risk screening, abnormal ultrasound, or patient preference.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.70249","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:21:04Z","timestamp":1789597264000},"page":"1788-1795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cell\u2010Free DNA Screening After Euploid Embryo Transfer: Concordance With Amniocentesis and Residual Aneuploidy Risk"],"prefix":"10.1002","volume":"46","author":[{"given":"Yao","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Ren Ji Hospital Shanghai Jiao Tong University School of Medicine  Shanghai 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