{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T17:39:56Z","timestamp":1782754796503,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004374","name":"Chongqing Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Discovery"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acute kidney injury (AKI) is a prevalent and serious clinical challenge with limited specific treatments.\n                    <jats:italic>Phyllanthus urinaria L<\/jats:italic>\n                    (\n                    <jats:italic>PU<\/jats:italic>\n                    ), a well\u2010regarded herbal medicine, possesses noted anti\u2010inflammatory, antiviral, and antitumor properties. Yet, research into its renal protective effects, particularly for cisplatin\u2010induced AKI, is markedly limited. Our study explores the therapeutic potential of\n                    <jats:italic>PU<\/jats:italic>\n                    against AKI triggered by cisplatin administration. Utilizing an AKI model induced by a substantial dose of cisplatin, we assessed renal function through histological examination (hematoxylin and eosin staining) and biochemical markers, including blood urea nitrogen (BUN) and serum creatinine (Scr). Our results demonstrated that\n                    <jats:italic>PU<\/jats:italic>\n                    may mitigate cisplatin's structural damage to renal tissue. Post\u2010treatment with\n                    <jats:italic>PU<\/jats:italic>\n                    , a significant reduction in pro\u2010inflammatory cytokines interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) was observed through quantitative polymerase chain reaction (PCR) analysis. Additionally, the expressions of apoptotic and anti\u2010apoptotic proteins B cell leukemia\/lymphoma two (Bcl\u20102), Bcl2\u2010Associated X protein (Bax), and cleaved\u2010caspase3 evaluated via Western blot and immunofluorescence indicated that\n                    <jats:italic>PU<\/jats:italic>\n                    could inhibit apoptosis in response to cisplatin injury. Oxidative stress markers, such as malondialdehyde (MDA) and superoxide dismutase (SOD), showed decreased MDA levels and elevated SOD activity following\n                    <jats:italic>PU<\/jats:italic>\n                    treatment, suggesting a reduction in oxidative damage. Furthermore, cisplatin\u2010induced downregulation of\n                    <jats:italic>NRF2<\/jats:italic>\n                    and\n                    <jats:italic>NQO1<\/jats:italic>\n                    was countered by\n                    <jats:italic>PU<\/jats:italic>\n                    , highlighting its restorative effect on antioxidant pathways as confirmed by Western blot and immunofluorescence. Collectively, our findings endorse\n                    <jats:italic>PU<\/jats:italic>\n                    's protective capability against cisplatin\u2010induced AKI, endorsing its therapeutic potential to counteract cisplatin's nephrotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/pdi3.70000","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T12:44:41Z","timestamp":1747313081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Effects of\n                    <i>Phyllanthus urinaria L<\/i>\n                    on Cisplatin\u2010Induced Acute Kidney Injury: Anti\u2010Inflammatory, Anti\u2010Apoptotic, and Anti\u2010Oxidative Action"],"prefix":"10.1002","volume":"3","author":[{"given":"Qitong","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianju","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlan","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7822-8684","authenticated-orcid":false,"given":"Guanghui","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8488-2967","authenticated-orcid":false,"given":"Deying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders Chongqing Key Laboratory of Structural Birth Defect and Reconstruction National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-018-0052-0"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)32563-2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882-019-1645-y"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.02140215"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00927.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-015-3934-7"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00631-7"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.08070715"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20123011"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-022-06851-6"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-021-00620-9"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1099856"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01109"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000736"},{"issue":"3","key":"e_1_2_11_16_1","first-page":"S43","article-title":"Cytoprotective 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