{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:50:40Z","timestamp":1774983040600,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T00:00:00Z","timestamp":1755993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T00:00:00Z","timestamp":1755993600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021MD703923"],"award-info":[{"award-number":["2021MD703923"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Discovery"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Currently, there are no reliable pediatric biomarkers for assessing the severity of asthma. Therefore, the study aimed to assess the potential of glucocorticoid\u2010induced TNFR\u2010related ligand (GITRL) in assessing asthma severity. Bone marrow\u2010derived dendritic cells (BMDCs) were isolated and treated with\u00a0phosphate\u2010buffered saline (PBS) or house dust mites (HDM). Flow cytometry, immunofluorescence, and RT\u2010qPCR results revealed that GITRL expression was increased in HDM\u2010treated BMDCs (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). An asthma mouse model was established using HDM. Asthmatic mice exhibited significantly increased lung inflammation scores, serum IgE, total cell counts, and eosinophil counts in bronchoalveolar lavage fluid (BALF) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Tissue immunofluorescence and immunohistochemistry indicated elevated GITRL expression levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.03). Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) isolated from children showed that the proportion of GITRL\u00a0+\u00a0dendritic cells (DCs) and the mean fluorescence intensity (MFI) of GITRL were significantly increased in asthmatic children compared with healthy individuals (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). RT\u2010qPCR analysis showed that\n                    <jats:italic>GITRL<\/jats:italic>\n                    mRNA expression levels were significantly elevated in children with moderate\u2010to\u2010severe asthma compared to those with mild asthma (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.006), and\n                    <jats:italic>GITRL<\/jats:italic>\n                    mRNA levels were higher in both groups of asthmatic children than in healthy controls\u00a0(\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Correlation analysis indicated that\n                    <jats:italic>GITRL<\/jats:italic>\n                    expression was negatively correlated with lung function and the Children's Asthma Control Test (C\u2010ACT) score (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). ROC analysis assessed its diagnostic value, with AUC values of 0.89 (asthma vs. healthy diagnosis) and 0.70 (distinguishing moderate\u2010to\u2010severe from mild asthma). These findings suggest that GITRL is an important biomarker for assessing asthma severity.\n                  <\/jats:p>","DOI":"10.1002\/pdi3.70020","type":"journal-article","created":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T12:55:50Z","timestamp":1756040150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucocorticoid\u2010Induced TNFR\u2010Related Ligand as a Biomarker for Diagnosis and Severity of Asthma in Children"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3350-380X","authenticated-orcid":false,"given":"Yuehan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinying","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinying","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and Disorders China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Engineering Research Center of Stem Cell Therapy Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Ministry of 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