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A multidisciplinary working group was established under the auspices of the Preterm Birth International Collaborative (Australasia Branch). The development process included a comprehensive literature review of databases, which identified 61 publications. Recommendations were formulated using the Grading of Recommendations, Assessment, Development and Evaluation system for evidence evaluation. A modified Delphi method was used to achieve consensus. The consensus establishes that neonatal LI presents with nonspecific gastrointestinal symptoms, requiring stool assessment for diagnosis. Screening tests include fecal reducing sugar or urinary galactose tests, and the diagnosis is confirmed through elimination testing while excluding mimics. Treatment prioritizes lactase\u2010supplemented breastfeeding; formula\u2010fed infants receive lactase or reduced\u2010lactose formulas. Probiotics with \u03b2\u2010galactosidase activity can be used as an adjuvant therapy, and evidence\u2010based lactase preparations are recommended, particularly for preterm infants. Treatment duration should be individualized, with a general minimum of 2\u00a0weeks; however, the routine use of lactose\u2010free formula to prevent LI in preterm infants is not recommended. This consensus provides practical evidence\u2010based guidance to standardize the diagnosis and management of neonatal LI, aiming to reduce variation in practice and improve patient outcomes. It emphasizes the importance of maintaining breastfeeding while incorporating lactase supplementation and highlights the need for further high\u2010quality research, particularly randomized controlled trials, to refine diagnostic criteria and therapeutic strategies for preterm and very preterm infants. The recommendations are intended to support clinicians in making informed decisions while promoting family\u2010centered care.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>International Practice Guidelines Registry Platform (PREPARE\u20102024CN606)<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pdi3.70044","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T04:58:49Z","timestamp":1774673929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preterm Birth International Collaborative Australasia Branch: Expert Consensus on Diagnosis and Treatment of Neonatal Lactose Intolerance (2025)"],"prefix":"10.1002","volume":"4","author":[{"given":"Zhengli","family":"Wang","sequence":"first","affiliation":[{"name":"Children\u2019s Hospital of Chongqing Medical 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