{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T10:44:44Z","timestamp":1782902684811,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82501925"],"award-info":[{"award-number":["82501925"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Discovery"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hypospadias is among the most frequent congenital malformations of the male genitalia. The expression of\n                    <jats:italic>V\u2010maf musculoaponeurotic fibrosarcoma oncogene homolog B<\/jats:italic>\n                    (\n                    <jats:italic>MAFB<\/jats:italic>\n                    ), a transcription factor within the androgen signaling pathway, is closely correlated with the etiology of this condition. From an epigenetic perspective, this study investigated the role of microRNA\u2010130a\u20103p (miR\u2010130a\u20103p) in regulating\n                    <jats:italic>MAFB<\/jats:italic>\n                    during hypospadias pathogenesis. Clinical foreskin samples from hypospadias children and the dioctyl phthalate (DEHP)\u2010induced mouse genital tubercle tissues were analyzed. The expression levels of miR\u2010130a\u20103p and\n                    <jats:italic>MAFB<\/jats:italic>\n                    were detected by qRT\u2010PCR and western blot. In vitro, miR\u2010130a\u20103p was modulated in HS68 cells; CCK\u20108, scratch assay, western blot, flow cytometry, and dual\u2010luciferase assay were used to assess its effects and targeting of\n                    <jats:italic>MAFB<\/jats:italic>\n                    . Our study demonstrated that miR\u2010130a\u20103p targeted and suppressed\n                    <jats:italic>MAFB<\/jats:italic>\n                    expression. This suppression inhibited cellular proliferation and migration and reduced\n                    <jats:italic>Vimentin<\/jats:italic>\n                    mRNA expression. Cell cycle distribution was disrupted, marked by Gap 1 phase increase and Synthesis phase reduction, and the cell apoptosis rate increased. Cyclin\u2010dependent kinase 2 (CDK2), Cyclin E1, and Proliferating Cell Nuclear Antigen (PCNA) were downregulated. miR\u2010130a\u20103p targets and inhibits\n                    <jats:italic>MAFB<\/jats:italic>\n                    expression, disrupting the normal processes of cell proliferation, migration, cell apoptosis, and cell cycle progression, ultimately leading to the development of hypospadias.\n                  <\/jats:p>","DOI":"10.1002\/pdi3.70062","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:53:59Z","timestamp":1782899639000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MicroRNA\u2010130a\u20103p Targets the Androgen\u2010Related Transcription Factor\n                    <i>MAFB<\/i>\n                    : Effects on Proliferation, Migration, Apoptosis, and Cell Cycle in Hypospadias"],"prefix":"10.1002","author":[{"given":"Jiaxin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4328-7630","authenticated-orcid":false,"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0977-7345","authenticated-orcid":false,"given":"Zhenmin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongsong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingguo","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlan","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianju","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9249-1366","authenticated-orcid":false,"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University National Clinical Research Center for Children and Adolescents' Health and Diseases Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Structural Birth Defect and Reconstruction  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2002.024034"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcu.20832"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2017.02.022"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2020.12.036"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/ju.0000000000001143"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dms002"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.06.012"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1413273111"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.006"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2017.01.001"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20225547"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13353"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.9858"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2020.10.006"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2024.101432"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1049\/syb2.12096"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114892"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1256965"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614610114"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253\u2010011\u20109454\u2010z"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202000926rr"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gim.2023.100927"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2017.185"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9976"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3629"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcq243"},{"issue":"2","key":"e_1_2_11_28_1","first-page":"90","article-title":"Sometimes It Takes Darkness to See the Light: Pitfalls in the Interpretation of Cell Proliferation Markers (Ki\u201067 and PCNA)","volume":"10","author":"Castilla C.","year":"2012","journal-title":"Skinmed"}],"container-title":["Pediatric Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pdi3.70062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pdi3.70062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pdi3.70062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:54:00Z","timestamp":1782899640000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pdi3.70062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":27,"alternative-id":["10.1002\/pdi3.70062"],"URL":"https:\/\/doi.org\/10.1002\/pdi3.70062","archive":["Portico"],"relation":{},"ISSN":["2835-558X","2835-5598"],"issn-type":[{"value":"2835-558X","type":"print"},{"value":"2835-5598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]},"assertion":[{"value":"2025-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70062"}}