{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T14:43:59Z","timestamp":1776091439725,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T00:00:00Z","timestamp":1561420800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571948"],"award-info":[{"award-number":["81571948"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7172075"],"award-info":[{"award-number":["7172075"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Investigation"],"published-print":{"date-parts":[[2019,6]]},"abstract":"Abstract<\/jats:title>Importance<\/jats:title>Regional clonal replacements of methicillin\u2010resistant Staphylococcus aureus<\/jats:italic> (MRSA<\/jats:styled-content>) are common. It is necessary to understand the clonal and drug resistance changes in specific areas.<\/jats:p><\/jats:sec>Objective<\/jats:title>To evaluate the clonal and drug resistance dynamics of MRSA<\/jats:styled-content> in Chinese children from 2010 to 2017.<\/jats:p><\/jats:sec>Methods<\/jats:title>MRSA<\/jats:styled-content> was isolated from patients in Beijing Children's Hospital from 2010 to 2013 and from 2016 to 2017. The molecular characteristics and antibiotic resistance were determined.<\/jats:p><\/jats:sec>Results<\/jats:title>In total, 211 MRSA<\/jats:styled-content> isolates were collected, and 104 isolates were classified as community\u2010associated MRSA<\/jats:styled-content> (CA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content>). ST<\/jats:styled-content>59\u2010SCC<\/jats:styled-content>mec <\/jats:italic>IV<\/jats:styled-content> was the most prevalent type in both CA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content> (65.4%) and healthcare\u2010 associated\u2010MRSA<\/jats:styled-content> (HA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content>) (46.7%). ST<\/jats:styled-content>239\u2010SCC<\/jats:styled-content>mec <\/jats:italic>III<\/jats:styled-content> accounted for 21.5% of all HA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content>, which were not detected in 2016, and only three isolates were detected in 2017. The pvl<\/jats:italic> gene carrying rate of CA<\/jats:styled-content>\u2010 MRSA<\/jats:styled-content> was significantly higher than that of HA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content> (42.3% vs. 29.0%, P<\/jats:italic> = 0.0456). Among CA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content>, resistance rate to all tested antibiotics excluding chloramphenicol remained stable over the periods of 2010\u20132013 and 2016\u20132017. HA<\/jats:styled-content>\u2010MRSA<\/jats:styled-content> displayed an overall trend of decreased resistance to oxacillin, gentamicin, tetracycline, ciprofloxacin, and rifampin, and increased resistance to chloramphenicol, consistent with the difference of antibiotic resistance patterns between ST<\/jats:styled-content>59\u2010SCC<\/jats:styled-content>mec <\/jats:italic>IV<\/jats:styled-content> and ST<\/jats:styled-content>239\u2010SCC<\/jats:styled-content>mec <\/jats:italic>III<\/jats:styled-content> isolates. Vancomycin minimal inhibitory concentration (MIC<\/jats:styled-content>) creep was found in the study period in all MRSA<\/jats:styled-content> and ST<\/jats:styled-content>59\u2010SCC<\/jats:styled-content>mec <\/jats:italic>IV<\/jats:styled-content> isolates.<\/jats:p><\/jats:sec>Interpretation<\/jats:title>ST<\/jats:styled-content>59\u2010SCC<\/jats:styled-content>mec <\/jats:italic>IV<\/jats:styled-content> has spread to hospitals and replaced the traditional ST<\/jats:styled-content>239\u2010SCC<\/jats:styled-content>mec <\/jats:italic>III<\/jats:styled-content> clone, accompanied by changes in drug resistance. Furthermore, vancomycin MIC<\/jats:styled-content> creep indicated that the rational use of antibiotics should be seriously considered.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ped4.12129","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T21:28:49Z","timestamp":1561498129000},"page":"72-80","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Clonal and drug resistance dynamics of methicillin\u2010resistant Staphylococcus aureus<\/i> in pediatric populations in China"],"prefix":"10.1002","volume":"3","author":[{"given":"Xin","family":"Yang","sequence":"first","affiliation":[{"name":"Pediatric Intensive Care Unit Beijing Children's Hospital Capital Medical University National Center for Children's Health Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingchao","family":"Liu","sequence":"additional","affiliation":[{"name":"Pediatric Intensive Care Unit Beijing Children's Hospital Capital Medical University National Center for Children's Health Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Pediatric Intensive Care Unit Beijing Children's Hospital Capital Medical University National Center for Children's Health Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suyun","family":"Qian","sequence":"additional","affiliation":[{"name":"Pediatric Intensive Care Unit Beijing Children's Hospital Capital Medical University National Center for Children's Health Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihu","family":"Yao","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Major Diseases in Children National Key Discipline of Pediatrics (Capital Medical University) National Clinical Research Center for Respiratory Diseases Beijing Key Laboratory of Pediatric 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