{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T13:23:46Z","timestamp":1766150626769,"version":"3.48.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Investigation"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Importance<\/jats:title>\n                    <jats:p>Understanding the dynamic interplay between gut microbiota development and bilirubin metabolism may provide new insights into the pathophysiology of neonatal jaundice. Identifying microbial taxa associated with bilirubin fluctuations could help inform early prediction and microbiota\u2010targeted interventions for hyperbilirubinemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the correlation between dynamic changes in the gut microbiota and bilirubin concentrations during the neonatal period.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bilirubin concentrations were monitored daily throughout the neonatal period. Fecal samples were collected from neonates on days 1, 3, 7, 14, 21, and 28 after birth. The composition of the gut microbiome was assessed by 16S rRNA gene amplicon sequencing of the fecal samples. Within\u2010subject, same\u2010day associations between transcutaneous bilirubin (TcB) and genus\u2010level abundance were quantified using a repeated\u2010measures correlation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Thirty neonates were included in the final analysis. Among the top\u201030 genera, six exhibited false discovery rate significant, same\u2010day within\u2010subject associations with TcB under the repeated\u2010measures correlation framework (|r\n                      <jats:sub>rm<\/jats:sub>\n                      | \u22650.30). Changes in the abundances of the genera\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sub>rm<\/jats:sub>\n                      = +0.416, 95% confidence interval [CI] 0.272\u20130.543,\n                      <jats:italic>P<\/jats:italic>\n                      = 2.084 \u00d7 10\n                      <jats:sup>\u22127<\/jats:sup>\n                      ;\n                      <jats:italic>P<\/jats:italic>\n                      \u2010adj = 3.126 \u00d7 10\n                      <jats:sup>\u22126<\/jats:sup>\n                      ) and\n                      <jats:italic>Rothia<\/jats:italic>\n                      (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sub>rm<\/jats:sub>\n                      = +0.340, 95% CI 0.187\u20130.476;\n                      <jats:italic>P<\/jats:italic>\n                      = 3.134 \u00d7 10\n                      <jats:sup>\u22125<\/jats:sup>\n                      ;\n                      <jats:italic>P<\/jats:italic>\n                      \u2010adj = 1.567 \u00d7 10\n                      <jats:sup>\u22124<\/jats:sup>\n                      ) were positively correlated with bilirubin concentrations throughout the neonatal period. In complementary cross\u2010sectional analyses centered on meconium, additional genus\u2013bilirubin correlations were identified for TcB measured on postnatal days 3\u20137 and for the neonatal TcB peak, with multiplicity controlled separately for each endpoint.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>A correlation was found between dynamic changes in the gut microbiome and bilirubin concentrations during the neonatal period. The identified genera might be potential markers or targets for intervention for neonatal jaundice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ped4.70032","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T08:06:06Z","timestamp":1764921966000},"page":"347-360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic relationships between bilirubin concentrations and the gut microbiota in the neonatal period: A pilot prospective cohort study"],"prefix":"10.1002","volume":"9","author":[{"given":"Zhongyuan","family":"Li","sequence":"first","affiliation":[{"name":"Medical School of Chinese PLA  Beijing China"},{"name":"Senior Department of Pediatrics, the Seventh Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, The First Medical Center Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Medical Center Chinese PLA General Hospital  Beijing China"},{"name":"Department of Pediatrics, Beijing Institute for Brain Disorders, Center for Brain Disorders Research Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3928\/19382359\u201020220407\u201002"},{"key":"e_1_2_6_3_1","first-page":"E4","article-title":"Neonatal hyperbilirubinemia: an evidence\u2010based approach","volume":"68","author":"Pace EJ","year":"2019","journal-title":"J Fam 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