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The <jats:italic><jats:styled-content style=\"fixed-case\">GSNOR<\/jats:styled-content>1<\/jats:italic> loss\u2010of\u2010function mutant <jats:italic>gsnor1\u20103<\/jats:italic> overaccumulates <jats:styled-content style=\"fixed-case\">GSNO<\/jats:styled-content> with inherent high <jats:italic>S<\/jats:italic>\u2010nitrosylation level and resistance to the oxidative stress inducer paraquat (1,1\u2032\u2010dimethyl\u20104,4\u2032\u2010bipyridinium dichloride). Here, we report the characterization of <jats:italic>dgl1\u20103<\/jats:italic> as a genetic suppressor of <jats:italic>gsnor1\u20103<\/jats:italic>. <jats:italic><jats:styled-content style=\"fixed-case\">DGL<\/jats:styled-content>1<\/jats:italic> encodes a subunit of the oligosaccharyltransferse (<jats:styled-content style=\"fixed-case\">OST<\/jats:styled-content>) complex which catalyzes the formation of <jats:italic>N<\/jats:italic>\u2010glycosidic bonds in <jats:italic>N<\/jats:italic>\u2010glycosylation. The fact that <jats:italic>dgl1\u20103<\/jats:italic> repressed the paraquat resistance of <jats:italic>gsnor1\u20103<\/jats:italic> meanwhile <jats:italic>gsnor1\u20103<\/jats:italic> rescued the embryo\u2010lethal and post\u2010embryonic development defect of <jats:italic>dgl1\u20103<\/jats:italic> reminded us the possibility that <jats:italic>S<\/jats:italic>\u2010nitrosylation and <jats:italic>N<\/jats:italic>\u2010glycosylation crosstalk with each other through co\u2010substrates. By enriching glycoproteins in <jats:italic>gsnor1\u20103<\/jats:italic> and mass spectrometry analysis, <jats:styled-content style=\"fixed-case\">TGG<\/jats:styled-content>2 (thioglucoside glucohydrolase2) was identified as one of co\u2010substrates with high degradation rate and elevated <jats:italic>N<\/jats:italic>\u2010glycosylation level in <jats:italic>gsnor1\u20103 ost3\/6<\/jats:italic>. The <jats:italic>S<\/jats:italic>\u2010nitrosylation and <jats:italic>N<\/jats:italic>\u2010glycosylation profiles were also modified in <jats:italic>dgl1\u20103<\/jats:italic> and <jats:italic>gsnor1\u20103<\/jats:italic>. Thereby, we propose a linkage between <jats:italic>S<\/jats:italic>\u2010nitrosylation and <jats:italic>N<\/jats:italic>\u2010glycosylation through co\u2010substrates.<\/jats:p>","DOI":"10.1002\/pld3.110","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T15:15:32Z","timestamp":1551366932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A new insight to explore the regulation between <i>S<\/i>\u2010nitrosylation and <i>N<\/i>\u2010glycosylation"],"prefix":"10.1002","volume":"3","author":[{"given":"Hu","family":"Du","sequence":"first","affiliation":[{"name":"Vegetable Research Institute Guangdong Academy of Agricultural Sciences Guangdong Key Laboratory for New Technology Research of Vegetables Guangzhou China"},{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lichao","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ni","family":"Zhan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinye","family":"Mu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianru","family":"Zuo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 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