{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:14:47Z","timestamp":1791270887700,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T00:00:00Z","timestamp":1577059200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["267205415"],"award-info":[{"award-number":["267205415"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Direct"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Regulation of iron (Fe) acquisition and homeostasis is critical for plant survival. In Arabidopsis, Fe deficiency\u2010induced bHLH039 forms a complex with the master regulator FIT and activates it to upregulate Fe acquisition genes. FIT is partitioned between cytoplasm and nucleus, whereby active FIT accumulates more in the nucleus than inactive FIT. At the same time, there is so far no information on the subcellular localization of bHLH039 protein and how it is controlled. We report here that the bHLH039 localization pattern changes depending on the presence of FIT in the cell. When expressed in cells lacking FIT, bHLH039 localizes predominantly in the cytoplasm, including cytoplasmic foci in close proximity to the plasma membrane. The presence of FIT enhances the mobility of bHLH039 and redirects the protein toward primarily nuclear localization, abolishing its accumulation in cytoplasmic foci. This FIT\u2010dependent change in localization of bHLH039 found in transient fluorescent protein expression experiments was confirmed in both leaves and roots of Arabidopsis transgenic plants, stably expressing hemagglutinin\u2010tagged bHLH039 in wild\u2010type or <jats:italic>fit<\/jats:italic> mutant background. This posttranslational mechanism for intracellular partitioning of Fe\u2010responsive transcription factors suggests a signaling cascade that translates Fe sensing at the plasma membrane to nuclear accumulation of the transcriptional regulators.<\/jats:p>","DOI":"10.1002\/pld3.190","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T12:27:53Z","timestamp":1577104073000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Mobility and localization of the iron deficiency\u2010induced transcription factor bHLH039 change in the presence of FIT"],"prefix":"10.1002","volume":"3","author":[{"given":"Ksenia","family":"Trofimov","sequence":"first","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumen","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Eutebach","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00fcsra","family":"Acaroglu","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga","family":"Mohr","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Bauer","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"},{"name":"Cluster of Excellence on Plant Sciences Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzvetina","family":"Brumbarova","sequence":"additional","affiliation":[{"name":"Institute of Botany Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-014-0180-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top90"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.047233"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.149930"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2014.11.004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.024315"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-003-0011-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00006"},{"key":"e_1_2_8_11_1","article-title":"Phospho\u2010mutant activity assays provide evidence for alternative phospho\u2010regulation pathways of the transcription factor FER\u2010LIKE IRON DEFICIENCY\u2010INDUCED TRANSCRIPTION FACTOR","author":"Gratz R.","year":"2019","journal-title":"New Phytologist"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.01.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msg088"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssr065"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.116244"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.09.061"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.10.062"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2017.06.014"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.00163"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-019-0400-5"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.084715"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2011.06.005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.183285"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.2004.04423.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11171-7"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00594"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04565.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.056481"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0476-9"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-007-0535-x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sss089"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16232"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvi031"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00721-3"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2008.26"}],"container-title":["Plant Direct"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpld3.190","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.190","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pld3.190","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T10:40:27Z","timestamp":1693651227000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pld3.190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/pld3.190"],"URL":"https:\/\/doi.org\/10.1002\/pld3.190","archive":["Portico"],"relation":{},"ISSN":["2475-4455","2475-4455"],"issn-type":[{"value":"2475-4455","type":"print"},{"value":"2475-4455","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"2019-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00190"}}