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One of the most popular uses is as a reporter gene for visualizing endogenous promoter activities within plant tissues. Despite this popularity, specific protocols for minimizing nonrepresentative staining patterns, including false negatives, in challenging tissue types are not common. This became a large issue during our work on dark\u2010grown <jats:italic>Arabidopsis<\/jats:italic> hypocotyls, and we set out to develop a protocol that would ensure accurate staining in a tissue that is biologically resistant to reagent penetration. Through extensive testing using a variety of constitutive and endogenous promoter::GUS fusion lines, we have developed an optimized GUS staining protocol that combines the use of acetone as a fixative, deliberate physical damage, and proper positive and negative controls to help ensure accurate staining along the hypocotyl while minimizing false negatives. Hopefully, our recommendations will allow for improved staining that more accurately reflects the true activity of cloned endogenous promoters and thus facilitate a more accurate understanding of promoter activity in <jats:italic>Arabidopsis<\/jats:italic> hypocotyls and other hard\u2010to\u2010stain tissues.<\/jats:p>","DOI":"10.1002\/pld3.367","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T10:34:20Z","timestamp":1644834860000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Fake news blues: A GUS staining protocol to reduce false\u2010negative data"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8994-8508","authenticated-orcid":false,"given":"Lauren K.","family":"Dedow","sequence":"first","affiliation":[{"name":"Department of Molecular, Cell and Developmental Biology University of California Los Angeles  Los Angeles CA 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