{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:05:37Z","timestamp":1753869937802,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019191","name":"College of Agriculture and Life Sciences, Texas A and M University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Direct"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Both annual and perennial sorghum biomass serve as important forage for ruminant animals around the world. Unfortunately, sorghum can produce hydrogen cyanide (HCN), which, if occurring in high enough concentrations, can be toxic or lethal to animals that consume it. The objectives of this study were to develop a fast and inexpensive colorimetric assay to measure the hydrogen cyanide potential (HCN\u2010P) as well as to compare this with existing visual assays while assessing the range of variation for HCN\u2010P among perennial and annual sorghum biomass. The HCN\u2010P of 100 sorghum lines derived from an interspecific hybridization program was determined over 2\u2009years (establishment and regrowth) using both visual and colorimetric assays. Visual assessment underestimated the HCN\u2010P and was less accurate than colorimetry. Repeatability for HCN\u2010P across all sampling dates was functionally zero in the visual assessment and low for the colorimetric assay. This was mostly explained by the significant pedigree\u2009\u00d7\u2009year interaction effects and growth stage. Growth stage substantially influenced HCN\u2010P, which should be considered when feeding animals on fresh forage.<\/jats:p>","DOI":"10.1002\/pld3.448","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T06:53:24Z","timestamp":1666421604000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3278-1887","authenticated-orcid":false,"given":"Shakirah","family":"Nakasagga","sequence":"first","affiliation":[{"name":"Department of Soil and Crop Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seth C.","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Soil and Crop Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William L.","family":"Rooney","sequence":"additional","affiliation":[{"name":"Department of Soil and Crop Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Barr","sequence":"additional","affiliation":[{"name":"Texas A&amp;M Veterinary Medical Diagnostic Laboratory  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pheona","family":"Nabukalu","sequence":"additional","affiliation":[{"name":"The Land Institute  Salina Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stan","family":"Cox","sequence":"additional","affiliation":[{"name":"The Land Institute  Salina Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5221-6378","authenticated-orcid":false,"suffix":"Jr.","given":"Leo","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Department of Horticulture Sciences University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.35.4.535"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjps79-185"},{"issue":"1","key":"e_1_2_7_4_1","first-page":"73","article-title":"Summer forage sorghum yield, protein and prussic acid contents as affected by plant density and nitrogen topdressing","volume":"6","author":"Bahrani M. 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