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Because plants are a major source of edible fats and oils, it is important to understand the structural organization of the plant htACCase, relative to its potential to regulate fatty acid biosynthesis in plant plastids. Moreover, unique to the plant htACCase, noncatalytic subunits called biotin attachment domain\u2013containing (BADC) proteins are important in the assembly of the holoenzyme, and they specifically interact with the <jats:sc>BC<\/jats:sc>CP and the biotin carboxylase (BC) subunits. We report herein NMR structural studies of the Arabidopsis BCCP isozymes (<jats:sc>BC<\/jats:sc>CP1 and BCCP2). We calculated the structure of C\u2010terminal domain of BCCP1 (K<jats:sub>200<\/jats:sub>\u2010P<jats:sub>280<\/jats:sub>) and explored structural changes in the BCCP1 protein upon its interactions with <jats:sc>BC<\/jats:sc> and BADC. The chemical shift perturbation experiments identified potential surface residues on the BCCP1 protein that may facilitate physical interactions between BC and BADC proteins. These studies indicate that the BADC protein interacts with a \u201cthumb\u201d\u2010like protrusion, which is a common structural feature of the bacterial and plant <jats:sc>BC<\/jats:sc>CPs, and thereby acts as a potential \u201ccap\u201d to facilitate the assembly of a BC\u2013BCCP\u2013BADC complex.<\/jats:p>","DOI":"10.1002\/pld3.70057","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T07:26:16Z","timestamp":1742714776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Solution Structure and NMR Chemical Shift Perturbations of the Arabidopsis BCCP1 Identify Intersubunit Interactions Potentially Involved in the Assembly of the Heteromeric Acetyl\u2010CoA Carboxylase"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-6554","authenticated-orcid":false,"given":"Kiran\u2010Kumar","family":"Shivaiah","sequence":"first","affiliation":[{"name":"Roy J. 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Carver Department of Biochemistry, Biophysics, and Molecular Biology Iowa State University  Ames Iowa USA"},{"name":"Center for Biorenewable Chemicals Iowa State University  Ames Iowa USA"},{"name":"Department of Biochemistry and Molecular Biology University of Georgia  Athens Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4672-7139","authenticated-orcid":false,"given":"Basil\u00a0J.","family":"Nikolau","sequence":"additional","affiliation":[{"name":"Roy J. 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