{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:54:17Z","timestamp":1786978457123,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Direct"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Low temperatures are a major environmental stress that limits plant growth and development. Understanding the molecular mechanism of cold tolerance is therefore essential for improving crop performance through molecular breeding. In this study, we focused on CISP, a small, previously uncharacterized protein, specifically induced in barley roots under low temperature conditions, and investigated its potential role in cold tolerance. Heterologous expression of CISP in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    enhanced late\u2010phase bacterial growth at low temperatures compared with the wild\u2010type strain. Similarly, overexpression of CISP in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:styled-content>\n                    , which lacks an apparent ortholog, improved seedling growth under low temperature conditions. These results suggest that CISP can promote growth of heterologous organisms used in this study at low temperature environments. To investigate its molecular function, we performed RNA chaperone assays using RNA beacons that tend to form stable secondary structures. CISP reduced the formation of RNA secondary structures and facilitated their destabilization, indicating RNA chaperone\u2013like activity. CISP is a basic, low\u2010molecular\u2010weight protein containing an intrinsically disordered region (IDR) at its N\u2010terminus but lacking canonical RNA\u2010binding domains such as the cold shock domain (CSD) or RNA recognition motif (RRM). Our findings therefore suggest that CISP may represent a previously uncharacterized type of RNA chaperone. This study provides new insights into the structure and function of CISP and its potential contribution to cold tolerance in barley.\n                  <\/jats:p>","DOI":"10.1002\/pld3.70179","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:50:23Z","timestamp":1781247023000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["CISP, an Intrinsically Disordered Cold\u2010Inducible Barley Protein, Functions as a Small RNA Chaperone"],"prefix":"10.1002","volume":"10","author":[{"given":"Yutaro","family":"Okumura","sequence":"first","affiliation":[{"name":"Graduate School of Science Nagoya City University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Maksudul","family":"Haque","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya City University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8829-7950","authenticated-orcid":false,"given":"Shin\u2010ichiro","family":"Kidou","sequence":"additional","affiliation":[{"name":"Graduate School of Science Nagoya City University  Nagoya Japan"},{"name":"Research Center for Biological Diversity Nagoya City University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007487\u2010w"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22385"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2007.07.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad430"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00344\u2010026\u201012071\u20109"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422\u2010025\u201001125\u20104"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.196"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.014472"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205774200"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.025791"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1303"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err101"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv362"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.210222"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)81906\u20106"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603168103"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.200328"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1443317"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.4.3.5445"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx044"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/17429145.2017.1308568"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac203096k"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139731"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010025\u201001952\u20108"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2024.02.001"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2017.04.011"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2018.1506681"}],"container-title":["Plant Direct"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.70179","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pld3.70179","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.70179","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T01:09:59Z","timestamp":1782436199000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pld3.70179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":28,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/pld3.70179"],"URL":"https:\/\/doi.org\/10.1002\/pld3.70179","archive":["Portico"],"relation":{},"ISSN":["2475-4455","2475-4455"],"issn-type":[{"value":"2475-4455","type":"print"},{"value":"2475-4455","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70179"}}