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Tissue sections and laser captured microdissected cells and secretory products were analyzed by MALDI\u2010MS and from the recovered protein profiles, over 400 different proteins were monitored. Over 50 of these, some of which have been identified, displayed regionalized behavior from caput to cauda within the epididymis. Combining the information obtained from high\u2010resolution imaging mass spectrometry and laser captured microdissection experiments, numerous proteins were localized within the epididymis at the cellular level. Furthermore, from the signal intensities observed in the different protein profiles organized in space, semiquantitative information for each protein was obtained.<\/jats:p>","DOI":"10.1002\/pmic.200300474","type":"journal-article","created":{"date-parts":[[2003,10,28]],"date-time":"2003-10-28T08:00:40Z","timestamp":1067328040000},"page":"2221-2239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":123,"title":["Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry"],"prefix":"10.1002","volume":"3","author":[{"given":"Pierre","family":"Chaurand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Fouch\u00e9court","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly B.","family":"DaGue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baogang J.","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Reyzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Claire","family":"Orgebin\u2010Crist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Caprioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,10,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(97)80151-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr990076h"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35015709"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64904-8"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200110)1:10<1200::AID-PROT1200>3.0.CO;2-Q"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"14A","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH709>3.0.CO;2-F","volume":"192","author":"Curran S.","year":"2000","journal-title":"J. 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