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The complete genome of the <jats:italic>L. monocytogenes<\/jats:italic> EGDe strain, belonging to the serogroup 1\/2a, has been sequenced and is comprised of 2853 open reading frames. The objective of the current study was to construct a two\u2010dimensional (2\u2010D) database of the proteome of this strain. The soluble protein fractions of the microorganism were recovered either in the mid\u2010log or in the stationary phase of growth at 37\u00b0C. These fractions were analyzed by 2\u2010D electrophoresis (2\u2010DE), using immobilized pH gradient strips of various pH values (3\u201310, 3\u20136, and 5\u20138) for the first\u2010dimensional separations and 12.5% acrylamide gels for sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE). 201 protein spots corresponding to 126 different proteins were identified by matrix assisted laser desorption\/ionization\u2010time of flight\u2010mass spectrometry (MALDI\u2010TOF\u2010MS). The 2\u2010DE maps presented here provide a first basis for further investigations of protein expression in <jats:italic>L. monocytogenes<\/jats:italic>. In this way, the comparison of proteome between cells in the exponential or stationary phase of growth at 37\u00b0C allowed us to characterize 161 variations in protein spot intensity, of which 38 were identified. Among the differentially expressed proteins were ribosomal proteins (RpsF, RplJ, and RpmE), proteins involved in cellular metabolism (GlpD, PdhD, Pgm, Lmo1372, Lmo2696, and Lmo2743) or in stress adaptation (GroES and ferritin), a fructose\u2010specific phosphotransferase enzyme IIB (Lmo0399) and different post\u2010translational modified forms of listeriolysin (LLO).<\/jats:p>","DOI":"10.1002\/pmic.200300841","type":"journal-article","created":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T17:50:54Z","timestamp":1092073854000},"page":"3187-3201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Two\u2010dimensional electrophoresis database of <b><i>Listeria monocytogenes<\/i><\/b> EGDe proteome and proteomic analysis of mid\u2010log and stationary growth phase 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