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Using two\u2010dimensional electrophoresis we have analyzed shifts in stress protein profiles in response to energy deprivation as a model of ischemic injury to kidneys. A group of chaperones and stress\u2010induced mitogen activated protein (MAP) kinases were analyzed. In addition to examining stress protein induction and phosphorylation we have also examined the mechanism of cytoprotection by heat shock protein 70 (Hsp70). Our results show that, of the different stress proteins examined, only binding protein (BiP) and Hsp70 were significantly induced upon energy deprivation. Other stress proteins, including Hsp27, calnexin, Hsp90 and ERp57 showed alterations in their phosphorylation profiles. Three different MAP kinases, namely p38, extracellular signal regulated kisase and c\u2010jun <jats:italic>N<\/jats:italic>\u2010terminal kinase (JNK) were activated in response to energy deprivation. While JNK activation was linked to apoptosis, activated\u2010p38 was involved in phosphorylation of Hsp27. Study of inhibitors of Hsp70 induction or pre\u2010induction of Hsp70 indicated that induced Hsp70 was involved in the suppression of JNK activation thereby inhibiting apoptotic cell death. Our results provide important insights into the flux in stress protein profiles in response to simulated ischemia and highlight the antiapoptotic, cytoprotective mechanism of Hsp70 action.<\/jats:p>","DOI":"10.1002\/pmic.200390065","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T11:02:19Z","timestamp":1049454139000},"page":"513-526","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Stress protein flux during recovery from simulated ischemia: Induced heat shock protein 70 confers cytoprotection by suppressing JNK activation and inhibiting apoptotic cell death"],"prefix":"10.1002","volume":"3","author":[{"given":"Yadunanda","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Utpal","family":"Tatu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,31]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Stress Proteins: Handbook of Experimental Pharmacology, Vol 136","author":"Latchman D.","year":"1998"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01171-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.243"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5427"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(00)01874-2"},{"key":"e_1_2_1_7_2","first-page":"F479","volume":"260","author":"Emami A.","year":"1991","journal-title":"Am. 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