{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:47:03Z","timestamp":1761058023262,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,8,11]],"date-time":"2004-08-11T00:00:00Z","timestamp":1092182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Web\u2010accessible proteome databases represent indispensable tools for quantitative and comparative proteomics research. The majority of two\u2010dimensional gel electrophoresis (2\u2010DE) databases contains clickable 2\u2010DE gel images and descriptive textual information such as protein name, <jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>\/p<jats:italic>I<\/jats:italic> values, methods of identification, cellular localization and other information on proteins. Although a great part of the work in comparative proteomics consists of the analysis of 2\u2010DE gels using image analysis approaches, most proteome databases lack the ability to present protein abundance data and their alterations within experiments <jats:italic>via<\/jats:italic> the web. Now, differentially regulated proteins detected in microbial experiments by quantitative gel image analysis are presented in a web\u2010accessible relational database DIFF (Differentially Regulated Proteins). The DIFF database is a part of the proteome database system for microbial research available at http:\/\/www.mpiib\u2010berlin.mpg.de\/2D\u2010PAGE.<\/jats:p>","DOI":"10.1002\/pmic.200400927","type":"journal-article","created":{"date-parts":[[2004,8,11]],"date-time":"2004-08-11T13:31:11Z","timestamp":1092231071000},"page":"2987-2990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Short Communication \u2013 Presentation of differentially regulated proteins within a web\u2010accessible proteome database system of microorganisms"],"prefix":"10.1002","volume":"4","author":[{"given":"Klaus\u2010Peter","family":"Plei\u00dfner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Schmelzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Wehrl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R.","family":"Jungblut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cfg.165"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300737"},{"key":"e_1_2_1_4_2","first-page":"32","volume":"1","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300396"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300483"},{"key":"e_1_2_1_7_2","unstructured":"http:\/\/us.expasy.org\/ch2d\/2d\u2010index.html"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300534"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01549.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150170505"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300074-MCP200"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2787(1997)16:3<145::AID-MAS2>3.0.CO;2-H"},{"key":"e_1_2_1_13_2"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300459"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300559"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200400927","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200400927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:46:44Z","timestamp":1761043604000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200400927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10]]},"references-count":14,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1002\/pmic.200400927"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200400927","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"type":"print","value":"1615-9853"},{"type":"electronic","value":"1615-9861"}],"subject":[],"published":{"date-parts":[[2004,10]]},"assertion":[{"value":"2004-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}