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These digestive enzymes were not detected in 60% of gastric cancer cases (18 out of 30 analyzed cases). Interestingly, an extraordinary amount of \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin was observed in these cases. In contrast to gastric cancer cases, \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin was detected in only 5% of patients (three out of 56) with chronic atrophic gastritis, and the detection frequency went up as the disease developed (one of four intestinal metaplasia cases, two of seven tubular adenoma cases, a single examined case of hyperplastic polyp and 60% of gastric cancer). Zymography showed that a 60\u00a0kDa protease strongly associated with \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin and mass spectrometric analysis revealed that the gastric \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin was a protease\u2010cleaved form. Our data suggest that \u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin and 60\u00a0kDa protease may serve as good diagnostic and prognostic markers for conditions associated with gastric cancer.<\/jats:p>","DOI":"10.1002\/pmic.200400960","type":"journal-article","created":{"date-parts":[[2004,9,20]],"date-time":"2004-09-20T08:02:24Z","timestamp":1095667344000},"page":"3343-3352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Proteomic analysis revealed a strong association of a high level of \u03b11\u2010antitrypsin in gastric juice with gastric cancer"],"prefix":"10.1002","volume":"4","author":[{"given":"Kyunghee","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minsu","family":"Kye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joung Soon","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ok Jae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taehyo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongbin","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,4]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000198218"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-148-38629"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.8.6.548"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.1999.0586"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7125(16)32071-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.31.3.279"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13975-X"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5011"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1514"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990601)20:7<1634::AID-ELPS1634>3.0.CO;2-J"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200201)2:1<112::AID-PROT112>3.0.CO;2-N"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200103)1:3<444::AID-PROT444>3.0.CO;2-Q"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181516"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080203"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990301)20:3<601::AID-ELPS601>3.0.CO;2-6"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.379"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1016-8478(23)15021-7","volume":"13","author":"Lee K.","year":"2002","journal-title":"Mol. 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