{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:33:32Z","timestamp":1789212812643,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T00:00:00Z","timestamp":1113782400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In two\u2010dimensional gel electrophoresis\u2010based proteomic experiments matrix\u2010assisted laser desorption\/ionization\u2010mass spectrometry (MALDI\u2010MS) peptide mass fingerprinting is often the technique of choice in identifying proteins. Here, we present a novel surface coating technique for MALDI\u2010MS targets that improves manual and automatic sample analysis. A mixture of silicone and graphite is spread in the form of a thin layer over the target. Due to the hydrophobicity of the coating, aqueous solutions can be applied to relatively small spots very precisely using a robotic system. At least four times more liquid can be concentrated on the same area compared to uncoated steel targets. \u03b1\u2010cyano\u20104\u2010hydrocinnamic acid crystallizes in form of very small crystals evenly distributed over the surface. The search for \u201chot spots\u201d during the analysis is not necessary, which supports the automatic acquisition of data. The homogeneous crystal layer can be very effectively washed on\u2010target without encountering major sample losses. This efficient washing and the focused application of aqueous samples replace expensive and time\u2010consuming reversed phase micro column based sample clean\u2010ups. When analyzing peptide mixtures, the signal intensities are up to five times higher than with preparations of the same un\u2010desalted samples on steel targets, since four times more sample can be loaded. The mass resolution remains unaffected by the surface coating. After usage the coating can be removed, followed by a new coating avoiding any carry\u2010over of sample to the next analysis. All these properties make the precoating of MALDI\u2010MS targets with a silicone\/graphite layer an ideal technique for routine analysis in large\u2010scale proteomic experiments.<\/jats:p>","DOI":"10.1002\/pmic.200401023","type":"journal-article","created":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T11:20:40Z","timestamp":1113823240000},"page":"1460-1471","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Silicone\/graphite coating for on\u2010target desalting and improved peptide mapping performance of matrix\u2010assisted laser desorption\/ionization\u2010mass spectrometry targets in proteomic experiments"],"prefix":"10.1002","volume":"5","author":[{"given":"Xinping","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Wilm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Franz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290020802"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00171a028"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2675315"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01510"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01511"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01107"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01111"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac001169y"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/415141a"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-1931(02)00206-9"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1101"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/422115a"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960799q"},{"key":"e_1_2_1_15_2","unstructured":"Langen H. 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