{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:49:53Z","timestamp":1791218993913,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T00:00:00Z","timestamp":1113782400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chlamydiae are widespread bacterial pathogens responsible for a broad range of diseases, including sexually transmitted infections, pneumonia and trachoma. To validate the existence of hitherto hypothetical proteins predicted from recent chlamydial genome sequencing projects and to examine the patterns of expression of key components at the protein level, we have surveyed the expressed proteome of <jats:italic>Chlamydia trachomatis<\/jats:italic> strain\u2005L2. A combination of two\u2010dimensional gel analysis, multi\u2010dimensional protein identification (MudPIT) and nanocapillary liquid chromatography\u2010tandem mass spectrometry allowed a total of 328\u2009chlamydial proteins to be unambiguously assigned. Proteins identified as being expressed in the metabolically inert form, elementary body, of <jats:italic>Chlamydia<\/jats:italic> include the entire set of predicted glycolytic enzymes, indicating that metabolite flux rather than <jats:italic>de novo<\/jats:italic> synthesis of this pathway is triggered upon infection of host cells. An enzyme central to cell wall biosynthesis was also detected in the intracellular form, reticulate body, of <jats:italic>Chlamydia<\/jats:italic>, suggesting that the peptidoglycan is produced during growth within host cells. Other sets of proteins identified include 17 outer membrane\u2010associated proteins of potential significance in vaccine studies and 67\u2009proteins previously annotated as hypothetical or conserved hypothetical. Taken together, \u226535% of the predicted proteome for <jats:italic>C.\u2005trachomatis<\/jats:italic> has been experimentally verified, representing the most extensive survey of any chlamydial proteome to date.<\/jats:p>","DOI":"10.1002\/pmic.200401044","type":"journal-article","created":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T11:22:52Z","timestamp":1113823372000},"page":"1558-1573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":97,"title":["Shotgun proteomic analysis of <b><i>Chlamydia trachomatis<\/i><\/b>"],"prefix":"10.1002","volume":"5","author":[{"given":"Paul","family":"Skipp","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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