{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:38:41Z","timestamp":1791117521284,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T00:00:00Z","timestamp":1108339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Difference in two\u2010dimensional (2\u2010D) gel electrophoresis (DIGE) is a novel method for analyzing up to three samples in one 2\u2010D gel and using the information gained to study post\u2010translational modifications of proteins. We describe the use of DIGE to isolate and characterize those proteins that undergo processing in spermatozoa as they transit the epididymal tract. We find up to 60 protein spots are significantly modified as sperm traverse the epididymis. In this article, we report eight unambiguous protein identifications and demonstrate that one protein, the \u03b2\u2010subunit of the mitochondrial F1\u2010ATPase, is serine\u2010phosphorylated as sperm undergo epididymal maturation. We suggest that phosphorylation of this particular protein in a cAMP\u2010dependent manner may contribute to the mechanisms by which motility is conferred upon spermatozoa.<\/jats:p>","DOI":"10.1002\/pmic.200401100","type":"journal-article","created":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T08:16:42Z","timestamp":1108369002000},"page":"1003-1012","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":107,"title":["Identification of post\u2010translational modifications that occur during sperm maturation using difference in two\u2010dimensional gel electrophoresis"],"prefix":"10.1002","volume":"5","author":[{"given":"Mark A.","family":"Baker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxanne","family":"Witherdin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Hetherington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Cunningham\u2010Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. John","family":"Aitken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,3,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"189","volume-title":"Mammalian Fertilization","author":"Yanagimachi R.","year":"1994"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/01485018308987488"},{"key":"e_1_2_1_4_2","first-page":"358","volume":"23","author":"Engel J. C.","year":"1973","journal-title":"Acta Physiol. Lat. Am."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb22266.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19880801"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080260104"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.5.956"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1790"},{"key":"e_1_2_1_10_2","first-page":"99","volume":"53","author":"Dacheux J. L.","year":"1998","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0690047"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(75)90037-0"},{"key":"e_1_2_1_13_2","first-page":"329","volume":"1","author":"Blaquier J. A.","year":"1974","journal-title":"Curr. Top. Mol. Endocrinol."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-3-839"},{"key":"e_1_2_1_15_2","first-page":"254","volume":"21","author":"Cameo M. S.","year":"1971","journal-title":"Acta Physiol. Lat. Am."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(79)90077-7"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(79)90098-0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.9.801"},{"key":"e_1_2_1_19_2","first-page":"149","volume":"53","author":"Frayne J.","year":"1998","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2003.11.010"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.5.1324"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1997.tb01895.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.4.917"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1242\/jcs.114.9.1787","volume":"114","author":"Zhu G. Z.","year":"2001","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230435"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019419"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181133"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01668.x"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300437"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390188"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T100007-MCP200"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300635"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00842"},{"key":"e_1_2_1_34_2","author":"Baker M. A.","year":"2003","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10255"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080290113"},{"key":"e_1_2_1_37_2","first-page":"183","volume":"53","author":"Temple\u2010Smith P. D.","year":"1998","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002524200"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199804)49:4<454::AID-MRD13>3.0.CO;2-U"},{"key":"e_1_2_1_40_2","author":"Asquith K. L.","year":"2004","journal-title":"Biol. Reprod."},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.6.2174"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202325200"},{"key":"e_1_2_1_43_2","author":"Ecroyd H.","year":"2003","journal-title":"Biol. Reprod."},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.3.1195"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0740105"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90426-7"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200401100","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200401100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:09:50Z","timestamp":1761062990000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200401100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1002\/pmic.200401100"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200401100","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3]]},"assertion":[{"value":"2004-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-03-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}