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Synovial biopsy samples were obtained from 18 patients undergoing needle arthroscopy for knee synovitis associated with RA (<jats:italic>n<\/jats:italic>\u2005=\u20056) and SpA (<jats:italic>n<\/jats:italic>\u2005=\u20056), and for joint effusion of the knee associated with OA (<jats:italic>n<\/jats:italic>\u2005=\u20056). Cytosolic proteins were extracted from the tissue and subjected to two\u2010dimensional gel electrophoresis. Protein expression patterns were statistically analyzed and used for hierarchical cluster analysis. Proteins of interest were independently identified by matrix\u2010assisted laser desorption\/ionization\u2010 and electrospray ionization\u2010mass spectrometry. Hierarchical cluster analysis of the complete match set, containing 640\u2005spots, remarkably segregated SpA from RA and OA. Next, we used a subset of spots that was statistically, differentially expressed (<jats:italic>P<\/jats:italic>\u2005&lt;\u20050.01), between RA and SpA, SpA and OA, or RA and OA, in both Student's <jats:italic>t<\/jats:italic>\u2010test and Mann\u2010Whitney <jats:italic>U<\/jats:italic>\u2010test. The dendrograms revealed distinct clustering of RA <jats:italic>versus<\/jats:italic> SpA and RA <jats:italic>versus<\/jats:italic> OA. Spots that were differentially expressed between the groups were identified by tandem mass spectrometry. Fructose bisphosphate aldolase A and alpha\u2010enolase showed higher expression levels in SpA than in OA (<jats:italic>P\u2005<\/jats:italic>&lt;\u20050.01). Calgranulin\u2005A myeloid related protein\u20108 (MRP\u20108) was markedly up\u2010regulated in RA and SpA patients in comparison to OA patients where this spot was below detection limit. The analysis of the cytosolic proteome of synovial tissue is a useful approach to identify disease\u2010associated proteins in chronic inflammatory arthritis.<\/jats:p>","DOI":"10.1002\/pmic.200401109","type":"journal-article","created":{"date-parts":[[2005,4,21]],"date-time":"2005-04-21T06:19:27Z","timestamp":1114064367000},"page":"2247-2257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Chronically inflamed synovium from spondyloarthropathy and rheumatoid arthritis investigated by protein expression profiling followed by tandem mass spectrometry"],"prefix":"10.1002","volume":"5","author":[{"given":"Kelly","family":"Tilleman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrien","family":"Van Beneden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Dhondt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Hoffman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"De Keyser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Veys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Elewaut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Deforce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,5,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210603"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0889-857X(21)00717-1","volume":"18","author":"Zeidler H.","year":"1992","journal-title":"Rheum. 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