{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:18:35Z","timestamp":1762100315260,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T00:00:00Z","timestamp":1108339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A mutation in the thyroglobulin (Tg) gene is the primary cause of hereditary dwarfism and hypothyroidism in the <jats:italic>rdw<\/jats:italic> rat. Despite the Tg mutation that causes a Tg shortage, <jats:italic>rdw<\/jats:italic> rats survive. The present study examines the influences of this condition on the pancreatic proteome. Normal control (group 1; <jats:italic>n<\/jats:italic> = 19) and <jats:italic>rdw<\/jats:italic> rats that did not receive L\u2010thyroxine (T<jats:sub>4<\/jats:sub>) (group 2; <jats:italic>n<\/jats:italic> = 27) were sacrificed from 4 to 56 weeks after birth. The <jats:italic>rdw<\/jats:italic> rats were supplemented either with daily intraperitoneal injections of T<jats:sub>4<\/jats:sub> from 3 to 28 days after birth (group 3; <jats:italic>n<\/jats:italic> = 4) or with normal thyroid tissues grafted at 4 weeks of age (group 4; <jats:italic>n<\/jats:italic> = 3). Groups 3 and 4 were sacrificed 12 weeks after birth. Pancreatic proteomes analyzed by two\u2010dimensional gel electrophoresis showed that levels of at least four pancreatic proteins were higher in group\u00a02 than in group 1, and that those of four were lower. Cluster decomposition and principal component analysis of the eight protein contents showed that groups\u00a01 and\u00a02 were separated into two clusters and that pancreatic proteomes of group\u00a04 were better normalized than those of group\u00a03. Injecting T<jats:sub>4<\/jats:sub> into group\u00a03 was temporarily effective, whereas the thyroid graft to group\u00a04 provided a continuous positive effect, which concurred with the increased body weight of the other two groups of <jats:italic>rdw<\/jats:italic> rats that received grafts of normal thyroids.<\/jats:p>","DOI":"10.1002\/pmic.200401117","type":"journal-article","created":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T08:16:58Z","timestamp":1108369018000},"page":"1113-1124","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effect of thyroxine on abnormal pancreatic proteomes of the hypothyroid <b><i>rdw<\/i><\/b> rat"],"prefix":"10.1002","volume":"5","author":[{"given":"Mamoru","family":"Satoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eri","family":"Haruta\u2010Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Omori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masamichi","family":"Oh\u2010Ishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Kodera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen\u2010Ichi","family":"Furudate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadakazu","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1538\/expanim1978.37.1_21","volume":"37","author":"Koto M.","year":"1988","journal-title":"Exp. 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