{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:06:07Z","timestamp":1774123567373,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2005,8,30]],"date-time":"2005-08-30T00:00:00Z","timestamp":1125360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Immunodepletion of albumin to improve the 2\u2010D gel resolution of human plasma proteins has recently been described. With the importance of mouse models in many studies in which serum or plasma is often analyzed, we have adopted this approach to immunoprecipitate mouse albumin and evaluated its effectiveness for 2\u2010D separation of mouse plasma proteins. Purified polyclonal antibodies against mouse albumin were effective depleting intact albumin as well as its numerous fragments from mouse plasma samples. Removal of albumin resulted in better resolution of mouse plasma proteins. Three proteins, \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    \u2010macroglobulin, coagulation factor XII, and hemopexin, that were previously either undetectable or poorly resolved, were identified from albumin\u2010depleted 2\u2010D gels by peptide mass fingerprinting. Albumin depletion also led to partial loss of several other proteins such as clusterin and gelsolin. This loss can be attributed to the interaction with albumin itself because the specificity of the antibody was demonstrated by Western blot. When applying this method to the 2\u2010D separation of plasma from inflamed mouse induced by cutaneous burn injury with superimposed\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    infection, the upregulation of inter \u03b1\u2010trypsin inhibitor heavy chain 4 (ITIH4) and hemopexin was unambiguously detected along with other mouse acute\u2010phase proteins (APP), including haptoglobin and serum amyloid A. Based on the significant increase of ITIH4, we propose that this protein is a new member of mouse APP that are upregulated during the inflammatory response.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200401257","type":"journal-article","created":{"date-parts":[[2005,8,30]],"date-time":"2005-08-30T04:46:46Z","timestamp":1125377206000},"page":"3991-4000","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Immunodepletion of albumin for two\u2010dimensional gel detection of new mouse acute\u2010phase protein and other plasma proteins"],"prefix":"10.1002","volume":"5","author":[{"given":"Xunbao","family":"Duan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Yarmush","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Berthiaume","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arul","family":"Jayaraman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin L.","family":"Yarmush","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,10]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Book Serum proteins in clinical medicine","author":"Ritchie R. 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