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In this study, a 2\u2010DE reference map and database of <jats:italic>B.\u2005anthracis<\/jats:italic>\u2005A16R was constructed. In total, 534\u2009spots were processed, and 406\u2009spots representing 299\u2009proteins were identified. Gel\u2010estimated p<jats:italic>I<\/jats:italic>s and molecular masses mostly matched well with their theoretical predictions, but some discrepancies also existed. Spot and protein corresponding analysis revealed that post\u2010translational modifications might be common in <jats:italic>B.\u2005anthracis<\/jats:italic>. Through the MASCOT search, the similarity of <jats:italic>B.\u2005anthracis<\/jats:italic>, <jats:italic>B.\u2005cereus<\/jats:italic> and <jats:italic>B.\u2005thuringiensis<\/jats:italic> was further verified by protein level and a possible annotation error in <jats:italic>B.\u2005anthracis<\/jats:italic> strain Ames\u20050581 genome was found. Proteins of energy metabolism, fatty acid and phospholipid metabolism, protein synthesis, and cellular processes represented a large part of the most abundant proteins. At the same time, 27\u2009hypothetical proteins were experimentally proved. There were 28\u2009proteins also identified as spore composition in recently spore\u2010related research, which indicated that they might play some roles in different phases such as growth, sporulation and outgrowth. Maps and information about all identified proteins are available on the Internet at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.mpiib-berlin.mpg.de\/2D-PAGE\">http:\/\/www.mpiib\u2010berlin.mpg.de\/2D\u2010PAGE<\/jats:ext-link> and <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.proteomics.com.cn\">http:\/\/www.proteomics.com.cn<\/jats:ext-link>.<\/jats:p>","DOI":"10.1002\/pmic.200401322","type":"journal-article","created":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T11:52:04Z","timestamp":1132228324000},"page":"4488-4495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["2\u2010D reference map of <b><i>Bacillus anthracis<\/i><\/b> vaccine strain A16R proteins"],"prefix":"10.1002","volume":"5","author":[{"given":"Junjun","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyi","family":"Ying","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoxing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erling","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihua","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofu","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houchu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peitang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4563-4579.2003"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(01)01050-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01586"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180807"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1187::AID-ELPS1187>3.0.CO;2-F"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00281458"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150090913"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01511"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300503"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150160123"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180728"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150190527"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150191046"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1128\/iai.65.11.4515-4524.1997","volume":"65","author":"Sonnenberg M.G.","year":"1997","journal-title":"Infect. 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