{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:53:22Z","timestamp":1761807202765,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2005,11,9]],"date-time":"2005-11-09T00:00:00Z","timestamp":1131494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of\n                    <jats:italic>S.\u2005flexneri<\/jats:italic>\n                    2a\u20052457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126\u2009marked spots were cut out and processed to MALDI\u2010TOF\u2010MS and PMF. Eighty\u2010seven spots were identified and they represented 55\u2005OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34\u2009immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.proteomics.com.cn\">www.proteomics.com.cn<\/jats:ext-link>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/pmic.200401326","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T15:26:43Z","timestamp":1132154803000},"page":"4777-4793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Immunoproteomics of outer membrane proteins and extracellular proteins of\n                    <b>\n                      <i>Shigella flexneri<\/i>\n                    <\/b>\n                    2a 2457T"],"prefix":"10.1002","volume":"5","author":[{"given":"Tianyi","family":"Ying","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoxing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erling","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiankai","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofu","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihua","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peitang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200105000-00011"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305519"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1128\/CDLI.5.4.537-542.1998","volume":"5","author":"McAtee C. P.","year":"1998","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00215-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200203)2:3<313::AID-PROT313>3.0.CO;2-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.915-920.2000"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(20000701)21:13<2670::AID-ELPS2670>3.0.CO;2-5"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4563-4579.2003"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150170130"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(20000701)21:13<2651::AID-ELPS2651>3.0.CO;2-3"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<1001::AID-ELPS1001>3.0.CO;2-L"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.01.038"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.1.97"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501501183"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200208)23:15<2373::AID-ELPS2373>3.0.CO;2-W"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01296.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04040.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200104)1:5<705::AID-PROT705>3.0.CO;2-N"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2775-2786.2003"},{"key":"e_1_2_1_21_2","first-page":"29","volume-title":"Escherichia coli and Salmonella: Cellular and Molecular Biology","author":"Nikaido H.","year":"1996"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1128\/iai.59.7.2252-2258.1991","volume":"59","author":"Weiser J. N.","year":"1991","journal-title":"Infect. Immunol."},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00383-3"},{"key":"e_1_2_1_24_2","first-page":"55","volume":"79","author":"Roy S.","year":"1994","journal-title":"Microbios"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1128\/jb.155.3.1434-1438.1983","volume":"155","author":"Higgins H. M. M.","year":"1983","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.5.1123-1128.1995"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.1884-1892.2000"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.25.15607"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00537-0"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200401326","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200401326","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:15:56Z","timestamp":1761066956000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200401326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12]]},"references-count":28,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["10.1002\/pmic.200401326"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200401326","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"type":"print","value":"1615-9853"},{"type":"electronic","value":"1615-9861"}],"subject":[],"published":{"date-parts":[[2005,12]]},"assertion":[{"value":"2004-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}