{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:41:57Z","timestamp":1791229317049,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,9]],"date-time":"2005-11-09T00:00:00Z","timestamp":1131494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    is a Gram\u2010negative anaerobic pathogen associated with chronic periodontitis. Although anaerobic,\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    exhibits a high degree of aerotolerance, which enables it to survive within periodontal pockets. The aim of the present study was to examine the effect of oxidative stress on protein expression in\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    to obtain a better understanding of the mechanism underlying its aerotolerance. To accomplish this,\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    cells were grown under conditions of hemin limitation (0.01\u2005\u03bcg\/mL) to avoid the oxygen protective effect of hemin on oxidative stress. The proteins were then extracted from cultures either left untreated or subjected to oxidative stress and separated by 2\u2010DE. The resultant protein expression profiles were examined by image scanning, and those found to differ depending on the presence or absence of aeration were subjected to MALDI\u2010MS and then analyzed using the ORF database of\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    W83 from The Institute of Genomic Research. Oxidative stress was found to affect the expression of numerous proteins in\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    cells. In particular, the levels of HtpG, GroEL, DnaK, AhpC, TPR domain protein, and trigger factor were substantially increased.\n                  <\/jats:p>","DOI":"10.1002\/pmic.200401338","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T15:27:04Z","timestamp":1132154824000},"page":"251-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Proteomics\u2010based analysis of a counter\u2010oxidative stress system in\n                    <b>\n                      <i>Porphyromonas gingivalis<\/i>\n                    <\/b>"],"prefix":"10.1002","volume":"6","author":[{"given":"Soichiro","family":"Okano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruaki","family":"Shiroza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimitsu","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1706-1714.2004"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.18.5591-5601.2003"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1600-0757.1999.tb00162.x","volume":"20","author":"Holt S. 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