{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:33:37Z","timestamp":1790249617303,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2005,10,11]],"date-time":"2005-10-11T00:00:00Z","timestamp":1128988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trichinella spiralis<\/jats:italic> is an intracellular nematode parasite of mammalian skeletal muscle. Infection of the muscle cell leads to the formation of a host\u2010parasite complex that results in profound alterations to the host cell and a re\u2010alignment of muscle\u2010specific gene expression. The role of parasite excretory\u2010secretory (ES) proteins in mediating these effects is currently unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, a global proteomics approach was used to analyse the ES\u2005proteins from <jats:italic>T.\u2005spiralis<\/jats:italic> muscle larvae. Following 2\u2010DE of ES\u2005proteins,MALDI\u2010TOF\u2010MS and LC\u2010MS\/MS were used to identify the peptide spots. Specific <jats:italic>Trichinella<\/jats:italic> EST databases were assembled and used to analyse the data. Despite the current absence of a <jats:italic>Trichinella<\/jats:italic> genome\u2010sequencing project, 43 out of 52\u2009protein spots analysed were identified and included the major secreted glycoproteins. Other novel proteins were identified from matches with sequences in the <jats:italic>T.\u2005spiralis<\/jats:italic> database. Our results demonstrate the value of proteomics as a tool for the identification of <jats:italic>Trichinella<\/jats:italic> ES\u2005proteins and in the study of the molecular mechanism underpinning the formation of the host\u2010parasite complex during <jats:italic>Trichinella<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/pmic.200402057","type":"journal-article","created":{"date-parts":[[2005,10,11]],"date-time":"2005-10-11T10:18:03Z","timestamp":1129025883000},"page":"4525-4532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["Proteomic analysis of the excretory\u2010secretory proteins of the <b><i>Trichinella spiralis<\/i><\/b> L1\u2009larva, a nematode parasite of skeletal muscle"],"prefix":"10.1002","volume":"5","author":[{"given":"Mark\u2005W.","family":"Robinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"Connolly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-3578-8_3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.4.785"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(91)90152-M"},{"key":"e_1_2_1_5_2","first-page":"477","volume":"78","author":"Despommier D.","year":"1975","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00015777"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200006042X"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4065-4071.2001"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00199-0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001008459"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099005879"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00174.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s00436-002-0815-0","volume":"90","author":"Lun H. M.","year":"2003","journal-title":"Parasitol. Res."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(92)90035-8"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00145-X"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3658-3662.2001"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4917-4924.2002"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00094-4"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212453200"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200109)1:9<1128::AID-PROT1128>3.0.CO;2-0"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(04)00306-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1061"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00287-0"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90782-6"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150121110"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh010"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/4.5.593"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.4049\/jimmunol.144.8.3152","volume":"144","author":"Denkers E. Y.","year":"1990","journal-title":"J. Immunol."},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90187-Q"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099004187"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00308-3"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2003)089[0092:MCACOA]2.0.CO;2"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.04.008"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90159-J"},{"key":"e_1_2_1_37_2","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1128\/IAI.66.5.1941-1945.1998","volume":"66","author":"McVay C. S.","year":"1998","journal-title":"Infect. Immun."},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.2307\/3281847"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.11.4806-4812.1997"},{"key":"e_1_2_1_40_2","first-page":"487","volume":"65","author":"Appleton J. A.","year":"1988","journal-title":"Immunology"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)90072-8"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200402057","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200402057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:46:23Z","timestamp":1761061583000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200402057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":40,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["10.1002\/pmic.200402057"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200402057","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11]]},"assertion":[{"value":"2004-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}