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In the current study, proteomic methodologies were applied to characterize functional outer membrane proteins (Omps) of <jats:italic>E.\u2005coli<\/jats:italic>\u2005K\u201012 responded to tetracycline and ampicillin resistance for understanding of universal pathways that form barriers for antimicrobial agents. For this purpose, <jats:italic>E.\u2005coli<\/jats:italic>\u2005K\u201012 expressional outer membrane proteome was characterized and identified with the use of 2\u2010DE and MALDI\u2010TOF\/MS methods. Then, differential Omps due to tetracycline or ampcilin resistance were determined by comparison between tetracycline minimum inhibitory concentration (MIC)<jats:sub>10<\/jats:sub>, ampicillin\u2005MIC<jats:sub>10<\/jats:sub>, control<jats:sub>0<\/jats:sub> and control<jats:sub>10<\/jats:sub>, showing 9\u2009proteins with 11\u2009spots for tetracycline and 8\u2009protein with 9\u2009spots for ampicillin, showing a difference in only 1\u2009protein (decreased LamB in tetracyclin) between the two antibiotics. Among the proteins, 3\u2009were known as antibiotic\u2010resistant proteins, including TolC, OmpC and YhiU, while FimD precursor, LamB, Tsx, YfiO, OmpW, NlpB were first reported here to be antibiotic\u2010resistance\u2010related proteins. Our findings will be helpful for further understanding of antibiotic\u2010resistant mechanism(s). This study also shows that the combination of Omp purification methods certainly contributes the sensitivity of Omp detection.<\/jats:p>","DOI":"10.1002\/pmic.200500219","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T10:49:48Z","timestamp":1135162188000},"page":"462-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":118,"title":["Analysis of outer membrane proteome of <b><i>Escherichia coli<\/i><\/b> related to resistance to ampicillin and tetracycline"],"prefix":"10.1002","volume":"6","author":[{"given":"Changxin","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixia","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanxian","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10915810305089"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-002-1019-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1187::AID-ELPS1187>3.0.CO;2-F"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/07853890109002073"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1055144"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153625"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90533-4"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"5803","DOI":"10.1128\/jb.178.19.5803-5805.1996","volume":"178","author":"Fralick J. 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