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We previously showed that FGF\/FGFR\u2010activated protein kinase\u2005C (PKC) is involved in the expression and activity of Runx2. Activated PKC\u03b4 physically interacts with Runx2 in FGF2\u2010stimulated MC3T3\u2010E1 preosteoblastic cells. Immunopurified Runx2 protein reacted with PKC\u03b4 kinase, and a phosphorylated 1460\u2010Da peptide fragment (amino acids\u2005241\u2013252, 1380\u2010Da) derived from Runx2 was also detected in MS analysis. Computer analysis predicted that Ser247 in this Runx2 can be a possible phosphorylation site by PKC\u03b4. We also showed that Runx2 activity after FGF stimulation correlates with the presence of the Runx2 Ser247 residue. The S247A (Ser\u00a0\u2192\u00a0Ala) mutation confers decreased transcriptional activity on a Runx2\u2010responsive promoter after FGF treatment.<\/jats:p>","DOI":"10.1002\/pmic.200500289","type":"journal-article","created":{"date-parts":[[2006,1,19]],"date-time":"2006-01-19T03:46:28Z","timestamp":1137642388000},"page":"1166-1174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Runx2 phosphorylation induced by fibroblast growth factor\u20102\/protein kinase\u2005C pathways"],"prefix":"10.1002","volume":"6","author":[{"given":"Byung\u2010Gyu","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Jung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye\u2010Jeong","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youn\u2010Jeong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won\u2010Joon","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u2010Jin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Mo","family":"Ryoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u2010Yoel","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1194-269"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0994-98"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0295-165"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0295-173"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0896-492"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1295-462"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.9.1845"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"5611","DOI":"10.1242\/dev.126.24.5611","volume":"126","author":"Iseki S.","year":"1999","journal-title":"Development"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80258-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200105052"},{"issue":"3","key":"e_1_2_1_13_2","first-page":"91","volume":"60","author":"Komori T.","year":"2002","journal-title":"Nippon Rinsho"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19970701)66:1<1::AID-JCB1>3.0.CO;2-V"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.11.6972"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80259-7"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203750200"},{"issue":"1","key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1080\/03008200390152188","volume":"44","author":"Franceschi R. 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