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Analyses of the 2\u2010DE protein profiles of the excretory\u2013secretory products (ESP) of adult <jats:italic>P. westermani<\/jats:italic> revealed approximately 147 protein spots, at least 15 of which were identified as cysteine proteases (CPs), at pHs between 4.5 and 8.5, and molecular weights (MWs) between 27 and 35\u2009kDa. An additional three CPs (designated as PwCP\u20103, \u20108 and \u201011) were newly recognized by TOF\/TOF MS. Their molecular biological information, which shared a high level sequence homology, was elucidated. The majority of the CPs reacted strongly with sera from paragonimiasis patients. When we observed the chronological changes in the antibody responses of the respective CPs against canine sera collected serially at 1, 3, 5, 7, 11 and 14\u2009wk after experimental infection, these molecules exhibited a multiplicity of distinct immune recognition patterns. Our results clearly showed that <jats:italic>P. westermani<\/jats:italic> adult ESP were principally composed of excretory\u2013secretory CPs, and that these CPs may exert effects not only on host tissue degradation and nutrient uptake, but also on the immune\u2010regulating cells <jats:italic>via<\/jats:italic> synergistic and independent interactions.<\/jats:p>","DOI":"10.1002\/pmic.200500399","type":"journal-article","created":{"date-parts":[[2006,1,10]],"date-time":"2006-01-10T14:25:58Z","timestamp":1136903158000},"page":"1290-1300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Identification of immunodominant excretory\u2013secretory cysteine proteases of adult <b><i>Paragonimus westermani<\/i><\/b> by proteome analysis"],"prefix":"10.1002","volume":"6","author":[{"given":"Eung\u2010Goo","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byoung\u2010Kuk","family":"Na","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010An","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon\u2010Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun\u2010Young","family":"Je","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Won","family":"Ju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Hyeong","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong\u2010Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Yong","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Yull","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60149-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-5231(01)00006-5"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiographics.13.3.8316665"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.162.1.8273653"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1259\/0007-1285-61-721-83"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.1880080209"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000064251"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000073264"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000077355"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/3283911"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60243-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00438-8"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1995.33.4.323"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.490_2.x"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1599-1604.2001"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.2307\/3284286"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4269\/ajtmh.2004.71.87","volume":"71","author":"Yang S. 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