{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T08:01:42Z","timestamp":1772784102853,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T00:00:00Z","timestamp":1137974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Niemann\u2010Pick disease type\u2005C (NPC) is a fatal autosomal recessive cholesterol disorder characterized by severe progressive neurodegeneration. To unveil the mechanism of neurodegeneration, proteomic and morphological approaches were applied to the hippocampus in NPC\u2005\u2013\/\u2013 mouse. Two\u2010DE was utilized to resolve the hippocampal protein expression profiles of 4\u2010 and 8\u2010week\u2010old NPC\u2005+\/+ and \u2013\/\u2013 mice. Differentially expressed protein spots were identified by MALDI\u2010TOF\u2005MS and database searching. At 4\u2009weeks of age, there was no significant difference in protein profiles between NPC\u2005+\/+ and \u2013\/\u2013 mice. However, at the age of 8\u2009weeks, NPC\u2005+\/+ and \u2013\/\u2013 mice showed marked difference in protein expressions. Among these, glutamate receptor\u20052 precursor was identified. The immunohistochemical study on neurotransporters showed that glial GABA transporter (GAT\u20103) increased in both 4\u2010 and 8\u2010week\u2010old NPC\u2005\u2013\/\u2013 mouse and glutamic acid decarboxylase (GAD\u20106) increased in 8\u2010week\u2010old NPC\u2005\u2013\/\u2013 mouse. Glial glutamate transporter, excitatory amino acids carrier\u20101 (EAAC1), decreased in 8\u2010week\u2010old NPC\u2005\u2013\/\u2013 mouse. In conclusion, our data may provide insight into the understanding of the basic mechanism through perturbation of protein networks and neurotransporter systems in a single gene knockout model of NPC disease.<\/jats:p>","DOI":"10.1002\/pmic.200500412","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T05:25:13Z","timestamp":1137993913000},"page":"1230-1236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Alteration of the glutamate and GABA transporters in the hippocampus of the Niemann\u2010Pick disease, type\u2005C mouse using proteomic 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