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While effective polysaccharides\u2010based vaccines exist against serogroups\u2005A, C, W135, and Y, no similar vaccine is suitable for children under 4\u2005years against disease caused by serogroup\u2005B strains. Therefore, major vaccine efforts against this serogroup are based on outer membrane vesicles (OMVs), containing major outer membrane proteins. The OMV\u2010based vaccine produced by the Finlay Institute in Cuba (VA\u2010MENGOC\u2010BC\u00ae) contributed to the rapid decline of the epidemic in this Caribbean island. While the content of major proteins in this vaccine has been discussed, no detailed work of an outer membrane proteomic map of this, or any other, commercially available OMV\u2010derived product has been published so far. Since OMVs exhibit a large bias toward a few major proteins and usually contain a high content of lipids, establishing the adequate conditions for high resolution, 2\u2010DE of this kind of preparation was definitely a technical challenge. In this work, 2\u2010DE and MS have been used to generate a proteomic map of this product, detailing the presence of 31 different proteins, and it allows the identification of new putative protective protein components it contains.<\/jats:p>","DOI":"10.1002\/pmic.200500502","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T05:29:32Z","timestamp":1146720572000},"page":"3389-3399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Outer membrane vesicles of the VA\u2010MENGOC\u2010BC\u00ae vaccine against serogroup B <b><i>of Neisseria meningitidis<\/i><\/b>: Analysis of protein components by two\u2010dimensional gel electrophoresis and mass spectrometry"],"prefix":"10.1002","volume":"6","author":[{"given":"Liliam","family":"Uli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lila","family":"Castellanos\u2010Serra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lazaro","family":"Betancourt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n","family":"Barber\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin","family":"Sotolongo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Guill\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolando","family":"Paj\u00f3n Feyt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/315158"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268898001848"},{"key":"e_1_2_1_4_2","first-page":"115","volume":"112","author":"Jefferies C.","year":"1999","journal-title":"N. 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