{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T10:06:24Z","timestamp":1783850784762,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,5,17]],"date-time":"2006-05-17T00:00:00Z","timestamp":1147824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Venezuelan scorpion <jats:italic>Tityus discrepans<\/jats:italic> is known to cause human fatalities. We describe the first complete proteomic analysis of its venom. By HPLC 58 different fractions were obtained and 205 different components were identified by MS analysis. Components having molecular masses from 272 to 57\u2005908\u2009amu were found. Forty homogeneous components had their N\u2010terminal amino acid sequence determined by Edman degradation, from which two new peptides named TdK2 and TdK3 (meaning <jats:italic>T. discrepans<\/jats:italic> (Td) K<jats:sup>+<\/jats:sup> channel toxins 2 and 3) were fully characterized. The first contains 34 amino acid residues with a molecular mass of 3451\u2009amu, and the second has 36 amino acids with 3832\u2009amu. Both peptides are tightly bound by three disulfide bridges. TdK2 was shown to block reversibly the Shaker B K<jats:sup>+<\/jats:sup>\u2010channel expressed heterologously in Sf9 cells. The systematic number assigned to TdK2 is \u03b1\u2010KTx\u201018.2 and that of TdK3 is \u03b1\u2010KTx\u201018.3. Comparative analysis of the amino acid sequences found suggests that this venom contains peptides highly similar to those that block K<jats:sup>+<\/jats:sup> channels, as well as those that modify the gating mechanisms of Na<jats:sup>+<\/jats:sup> channels, found in other scorpions. Additionally, peptides similar to defensins were also identified.<\/jats:p>","DOI":"10.1002\/pmic.200500525","type":"journal-article","created":{"date-parts":[[2006,5,17]],"date-time":"2006-05-17T04:57:57Z","timestamp":1147841877000},"page":"3718-3727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":87,"title":["Proteomic analysis of <b><i>Tityus discrepans<\/i><\/b> scorpion venom and amino acid sequence of novel toxins"],"prefix":"10.1002","volume":"6","author":[{"given":"Cesar V. 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