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The chip was developed for the ArrayTube\u2122 platform providing a cheap and easy to handle technology solution that combines a microtube\u2010integrated protein chip with the classical procedure of a sandwich\u2010enzyme\u2010linked immunosorbent assay and signal amplification by streptavidin\u2010poly\u2010horseradish peroxidase. Specific immunoassays for <jats:italic>Staphylococcus<\/jats:italic> enterotoxin\u2005B, ricin, Venezuelan equine encephalitis virus, St.\u2005Louis encephalitis virus, West Nile virus, Yellow fever virus, Orthopox virus species, <jats:italic>Francisella tularensis<\/jats:italic>, <jats:italic>Yersinia pestis<\/jats:italic>, <jats:italic>Brucella melitensis<\/jats:italic>, <jats:italic>Burkholderia mallei<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> EHEC\u2005O157:H7 were developed and optimized. All assays could be completed within 1 to 1\u2005<jats:sup>1<\/jats:sup>\/<jats:sub>2<\/jats:sub>\u2005h and detection levels were demonstrated to be as low as in well established ELISAs. Most interesting, as a result of careful antibody screening and testing, it is currently possible to analyse at least five of the \u201cdirty dozen\u201d agents on one single protein chip in parallel.<\/jats:p>","DOI":"10.1002\/pmic.200500721","type":"journal-article","created":{"date-parts":[[2006,4,18]],"date-time":"2006-04-18T14:08:34Z","timestamp":1145369314000},"page":"2972-2981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["A simple and rapid protein array based method for the simultaneous detection of biowarfare agents"],"prefix":"10.1002","volume":"6","author":[{"given":"Birgit","family":"Huelseweh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Ehricht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Juergen","family":"Marschall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,11]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Protein Microarrays","author":"W\u00f6lfl S.","year":"2004"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000045653.92719.a4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1139"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300610"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00069-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1024-1031.2005"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.09.005"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2291-2302.2005"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1166\/gl.2003.029"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1989.tb00650.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1128\/jcm.29.1.131-137.1991","volume":"29","author":"Greiser\u2010Wilke I. 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