{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:20:35Z","timestamp":1778642435374,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T00:00:00Z","timestamp":1149033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Brucella abortus<\/jats:italic> is the etiologic agent of bovine brucellosis and causes a chronic disease in humans known as undulant fever. In livestock the disease is characterized by abortion and sterility. Live, attenuated vaccines such as S19 and RB51 have been used to control the spread of the disease in animals; however, they are considered unsafe for human use and they induce abortion in pregnant cattle. For the development of a safer and equally efficacious vaccine, immunoproteomics was utilized to identify novel candidate proteins from <jats:italic>B.\u2005abortus<\/jats:italic> cell envelope\u2005(CE). A total of 163\u2005proteins were identified using 2\u2010DE with MALDI\u2010TOF\u2005MS and LC\u2010MS\/MS. Some of the major protein components include outer\u2010membrane protein (OMP)\u200525, OMP31, Omp2b porin, and 60\u2005kDa chaperonin GroEL. 2\u2010DE Western blot analyses probed with antiserum from bovine and a human patient infected with <jats:italic>Brucella<\/jats:italic> identified several new immunogenic proteins such as fumarate reductase flavoprotein subunit, F0F1\u2010type ATP synthase\u2005\u03b1 subunit, and cysteine synthase\u2005A. The elucidation of the immunome of <jats:italic>B.\u2005abortus<\/jats:italic> CE identified a number of candidate proteins for developing vaccines against <jats:italic>Brucella<\/jats:italic> infection in bovine and humans.<\/jats:p>","DOI":"10.1002\/pmic.200500730","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T17:15:38Z","timestamp":1149095738000},"page":"3767-3780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Proteomic analysis of <b><i>Brucella abortus<\/i><\/b> cell envelope and identification of immunogenic candidate proteins for vaccine development"],"prefix":"10.1002","volume":"6","author":[{"given":"Joseph P.","family":"Connolly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Comerci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy G.","family":"Alefantis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Walz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Quan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Chafin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Grewal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar V.","family":"Mujer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vito G.","family":"DelVecchio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_2_1_3_2","first-page":"339","volume-title":"Topley and Wilson's Principles of Bacteriology, Virology and Immunology","author":"Corbel M. J.","year":"1990"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00118-1"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.2.283"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0504.990411"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2003.11.001"},{"key":"e_1_2_1_8_2","first-page":"19","volume":"1","author":"Moriyon I.","year":"1998","journal-title":"Int. Microbiol."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.5.1929-1934.2006"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192319099"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8353-8361.2005"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180128"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"5274","DOI":"10.1128\/iai.64.12.5274-5283.1996","volume":"64","author":"Rafie\u2010Kolpin M.","year":"1996","journal-title":"Infect. Immun."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200208)2:8<1047::AID-PROT1047>3.0.CO;2-8"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.18.4962-4970.2002"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.6.1060"},{"key":"e_1_2_1_18_2","first-page":"283","volume-title":"Vaccination","author":"Nicoletti P.","year":"1990"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3611::AID-ELPS3611>3.0.CO;2-4"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01896.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-4-1115"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac991444k"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac001332p"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300074-MCP200"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti057"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/iai.64.9.3744-3751.1996","volume":"64","author":"Vizca\u00edno N.","year":"1996","journal-title":"Infect. Immun."},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1128\/iai.61.9.3785-3790.1993","volume":"61","author":"Marquis H.","year":"1993","journal-title":"Infect. Immun."},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.embor921"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200204144"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.080077197"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200410348"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27799-0"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02510.x"},{"key":"e_1_2_1_36_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/jb.73.2.211-217.1957","volume":"73","author":"Elberg S. S.","year":"1957","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(96)01110-5"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90091-S"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)93317-3"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(96)00018-7"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3286-3289.2000"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4857-4861.2003"},{"key":"e_1_2_1_43_2","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1128\/IAI.67.2.986-988.1999","volume":"67","author":"O\u00f1ate A. A.","year":"1999","journal-title":"Infect. Immun."},{"key":"e_1_2_1_44_2","first-page":"456","volume":"3","author":"Krah A.","year":"2003","journal-title":"Int. J. Cancer"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.1.5.765"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200500730","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200500730","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200500730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:52:42Z","timestamp":1761040362000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200500730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6,30]]},"references-count":44,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1002\/pmic.200500730"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200500730","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6,30]]},"assertion":[{"value":"2005-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}