{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T12:37:13Z","timestamp":1768739833316,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T00:00:00Z","timestamp":1145836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Pseudomonas putida<\/jats:italic>\u2005KT2440 is often used as a model to investigate toxicity mechanisms and adaptation to hazardous chemicals in bacteria. The objective of this paper was to test the impact of the chlorophenoxy herbicides 2,4\u2010dichlorophenoxyacetic acid\u2005(2,4\u2010D) and 2\u2010(2,4\u2010dichlorophenoxy)propanoic acid\u2005(DCPP) and their metabolites 2,4\u2010dichlorophenol\u2005(DCP) and 3,5\u2010dichlorocatechol\u2005(DCC), on protein expression patterns and physiological parameters. Both approaches showed that DCC has a different mode of action and induces different responses than DCPP, 2,4\u2010D and DCP. DCC was the most toxic compound and was active as an uncoupler of oxidative phosphorylation. It repressed the synthesis of ferric uptake regulator (Fur)\u2010dependent proteins, <jats:italic>e.g.<\/jats:italic> fumarase\u2005C and <jats:sc>L<\/jats:sc>\u2010ornithine N5\u2010oxygenase, which are involved in oxidative stress response and iron uptake. DCPP, 2,4\u2010D and DCP were less toxic than DCC. They disturbed oxidative phosphorylation to a lesser extent by a yet unknown mechanism. Furthermore, they repressed enzymes of energy\u2010consuming biosynthetic pathways and induced membrane transporters for organic substrates. A TolC homologue component of multidrug resistance transporters was found to be induced, which is probably involved in the removal of lipophilic compounds from membranes.<\/jats:p>","DOI":"10.1002\/pmic.200500781","type":"journal-article","created":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T12:53:47Z","timestamp":1145883227000},"page":"3319-3329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["<b><i>Pseudomonas putida<\/i><\/b> KT2440 responds specifically to chlorophenoxy herbicides and their initial metabolites"],"prefix":"10.1002","volume":"6","author":[{"given":"Dirk","family":"Benndorf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Thiersch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Loffhagen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christfried","family":"Kunath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hauke","family":"Harms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00370.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2000.tb00684.x"},{"key":"e_1_2_1_4_2","first-page":"557","volume":"508","author":"Cross J. 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